Fluorescence detection of coralyne and polyadenylation reaction using an oligonucleotide-based fluorogenic probe

被引：23
|
作者：
Lin, Yen-Hsiu [1 ]
Tseng, Wei-Lung [1 ]
机构：
[1] Natl Sun Yat Sen Univ, Dept Chem, Kaohsiung 80424, Taiwan
来源：
CHEMICAL COMMUNICATIONS | 2011年 / 47卷 / 39期
关键词：
ADENINE DNA DUPLEX; SYBR-GREEN-I; SMALL-MOLECULE; COLORIMETRIC DETECTION; BINDING; DISPROPORTIONATION; INTERCALATION; NANOPARTICLES; PALMATINE; HG2+;
D O I：
10.1039/c1cc14429d
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
This study describes the development of a simple, sensitive, and selective-detection system for coralyne and polyadenylation reaction based on the fact that coralyne induces a conformational change of the polyadenosine [poly(A)] oligonucleotide through A-coralyne-A coordination, thereby enhancing the fluorescence of SYBR Green I.
引用
收藏
页码：11134 / 11136
页数：3
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