(+)-pinoresinol-O--D-glucopyranoside from Eucommia ulmoides Oliver and its anti-inflammatory and antiviral effects against influenza A (H1N1) virus infection

被引：15

作者：

Li, Jing ^{[1
]}

Liang, Xiaoli ^{[1
]}

Zhou, Beixian ^{[2
]}

Chen, Xiaowei ^{[1
]}

Xie, Peifang ^{[1
]}

Jiang, Haiming ^{[1
]}

Jiang, Zhihong ^{[3
]}

Yang, Zifeng ^{[1
,3
]}

Pan, Xiping ^{[4
]}

机构：

[1] Guangzhou Med Univ, Guangzhou Inst Resp Hlth, Natl Clin Ctr Resp Dis, State Key Lab Resp Dis,Affiliated Hosp 1, 151 Yanjiang Xi Rd, Guangzhou 510120, Guangdong, Peoples R China

[2] Peoples Hosp Gaozhou, Dept Pharm, Gaozhou 525200, Guangdong, Peoples R China

[3] Macau Univ Sci & Technol, Macau Inst Appl Res Med & Hlth, State Key Lab Qual Res Chinese Med, Taipa 999078, Macau, Peoples R China

[4] Guangzhou Med Univ, Inst Chinese Integrat Med, 195 Dongfeng Xi Rd, Guangzhou 511436, Guangdong, Peoples R China

来源：

MOLECULAR MEDICINE REPORTS | 2019年 / 19卷 / 01期

关键词：

lignin; (+)-pinoresinol-O--D-glucopyranoside; influenza A virus; antiviral; anti-inflammatory; ACTIVATED PROTEIN-KINASE; COX-2; EXPRESSION; P38; MAPK; H5N1; HYPERCYTOKINEMIA; HEMAGGLUTININ; PATHOGENESIS; INHIBITION; INDUCTION; APOPTOSIS;

D O I：

10.3892/mmr.2018.9696

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Eucommia ulmoides Oliver (Du-Zhong) is an ancient Chinese herbal remedy used for the treatment of various diseases. To date, the effects of its constituent lignans on influenza viruses remain to be elucidated. In the present study, a lignan glycoside was isolated and purified from Eucommia ulmoides Oliver. Its structures were identified via extensive spectroscopic analysis, and its antiviral and anti-inflammatory activities, specifically against influenza viruses, were determined via a cytopathic effect (CPE) assay, plaque-reduction assays, a progeny virus yield reduction assay, reverse transcription-quantitative polymerase chain reaction analysis and a Luminex assay. Additionally, western blot analysis was performed to investigate the underlying mechanisms of its effects against influenza viruses. The chemical and spectroscopic methods determined the structure of lignan glycoside to be (+)-pinoresinol-O--D-glucopyranoside. The CPE assay showed that (+)-pinoresinol-O--D-glucopyranoside exerted inhibitory activities with 50% inhibition concentration values of 408.81 +/- 5.24 and 176.24 +/- 4.41 mu g/ml against the influenza A/PR/8/34 (H1N1) and A/Guangzhou/GIRD07/09 (H1N1) strains, respectively. Its antiviral properties were confirmed by plaque reduction and progeny virus yield reduction assays. Additional mechanistic analyses indicated that the anti-H1N1 virus-induced effects of (+)-pinoresinol-O--D-glucopyranoside were likely due to inactivation of the nuclear factor-B, p38 mitogen-activated protein kinase and AKT signaling pathways. Furthermore, (+)-pinoresinol-O--D-glucopyranoside exhibited pronounced inhibitory effects on the expression of influenza H1N1 virus-induced pro-inflammatory mediators, including tumor necrosis factor-, interleukin (IL)-6, IL-8 and monocyte chemoattractant protein 1. The data obtained suggest that (+)-pinoresinol-O--D-glucopyranoside may be a candidate drug for treating influenza H1N1 virus infection.

引用

页码：563 / 572

页数：10