Motility, ATP levels and metabolic enzyme activity of sperm from bluegill (Lepomis macrochirus)

被引:66
作者
Burness, G [1 ]
Moyes, CD
Montgomerie, R
机构
[1] Trent Univ, Dept Biol, Peterborough, ON K9J 7B8, Canada
[2] Queens Univ, Dept Biol, Kingston, ON K7L 3N6, Canada
来源
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY | 2005年 / 140卷 / 01期
基金
加拿大自然科学与工程研究理事会;
关键词
motility; enzyme; ATP; energy metabolism; sperm competition; fish; life history tactic;
D O I
10.1016/j.cbpb.2004.09.021
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Male bluegill displays one of two life history tactics. Some males (termed "parentals") delay reproduction until ca. 7 years of age, at which time they build nests and actively courts females. Others mature precociously (sneakers) and obtain fertilizations by cuckolding parental males. In the current study, we studied the relations among sperm motility, ATP levels, and metabolic enzyme activity in parental and sneaker bluegill. In both reproductive tactics, sperm swimming speed and ATP levels declined in parallel over the first 60 s of motility. Although sneaker sperm initially had higher ATP levels than parental sperm, by similar to30 s postactivation, no differences existed between tactics. No differences were noted between tactics in swimming speed, percent motility, or the activities of key metabolic enzymes, although sperm from parentals had a higher ratio of creatine phosphokinase (CPK) to citrate synthase (CS). In both tactics, with increasing CPK and CS activity, sperm ATP levels increased at 20 s postactivation, suggesting that capacities for phosphocreatine hydrolysis and aerobic metabolism may influence interindividual variation in rates of ATP depletion. Nonetheless, there was no relation between sperm ATP levels and either swimming speed or percent of sperm that were motile. This suggests that interindividual variation in ATP levels may not be the primary determinant of variation in sperm swimming performance in bluegill. (C) 2004 Elsevier Inc. All rights reserved.
引用
收藏
页码:11 / 17
页数:7
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