Micropropagation of Acacia seyal Del. in vitro

In vitro mass propagation of Acacia seyal Del. was achieved using seedling shoot tip explants on Murashige and Skoog (MS) medium supplemented with 6-benzyladenine (BA) or Thidiazuron (TDZ) with proportional to -Naphtalene acetic acid (NAA). The best result was obtained with BA in the presence of NAA. The greatest shoot multiplication with long shoots was observed on media containing (mg l(-1)) 2.0 BA with 0.1 or 0.5 NAA and 4.0 BA with 0.1 NAA; with 6.4 and 6.7 mean number of shoots, respectively. TDZ also induced multiple shoots but most of the shoots were stunted. Microshoots were rooted better on half-MS salts supplemented with Indole-3-butyric acid (IBA). The highest rooting percentage (80%) and root number (4.9 roots/microshoot) were promoted by 4.0 mg IBA. The plantlets (90%) successfully survived acclimatization ex vitro. (C) 2000 Academic Press.