Simultaneous and cost-effective determination of ethylene glycol and glycolic acid in human serum and urine for emergency toxicology by GC-MS

被引：12

作者：

Hlozek, Tomas ^{[1
,2
]}

Bursova, Miroslava ^{[1
,2
,3
]}

Cabala, Radomir ^{[1
,2
,3
]}

机构：

[1] Charles Univ Prague, Fac Med 1, Inst Forens Med & Toxicol, Prague 12108 2, Czech Republic

[2] Gen Univ Hosp, Prague 12108 2, Czech Republic

[3] Charles Univ Prague, Fac Sci, Dept Analyt Chem, Prague 12843 2, Czech Republic

来源：

CLINICAL BIOCHEMISTRY | 2015年 / 48卷 / 03期

关键词：

Glycols; Glycolic acid; Chloroformate; Toxicology; GC-MS; QUINTESSENTIAL CLINICAL TOXICOLOGY; ANALYTICAL CONUNDRUM;

D O I：

10.1016/j.clinbiochem.2014.12.002

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

Objectives: A simple, cost-effective, and fast gas chromatography method with mass spectrometry detection (GC-MS) for simultaneous measurement of ethylene glycol, 1,2-propylene glycol and glycolic acid was developed and validated for clinical toxicology purposes. Design and methods: Successful derivatization of glycolic acid with isobutyl chloroformate was achieved directly in serum/urine while glycols are simultaneously derivatized by phenylboronic acid. The entire sample preparation procedure is completed within 10 min. Results: The assay was proved to be quadratic in the range of 50 to 5000 mg L-1 with adequate accuracy (96.3-105.8%) and precision (CV <= 8.9%). Conclusion: The method was successfully applied to quantify the selected compounds in serum of patients from emergency units and the results correlated well with parallel GC-FID measurements (R-2 0.9933 for ethylene glycol and 0.9943 for glycolic acid). (C) 2014 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.

引用

页码：189 / 191

页数：3

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