Molecular characterization of Entamoeba histolytica RNase III and AGO2, two RNA interference hallmark proteins

被引：28

作者：

Abed, M ^{[1
]}

Ankri, S ^{[1
]}

机构：

[1] Technion Israel Inst Technol, Bruce Rappaport Fac Med, Dept Mol Microbiol, IL-31096 Haifa, Israel

来源：

EXPERIMENTAL PARASITOLOGY | 2005年 / 110卷 / 03期

关键词：

RNAi; RNA interference; siRNA; small interfering RNA; dsRNA; double strand RNA; Gal4AD; Gal4 activation domain; Gal4l3D. Ga14 DNA binding domain; Entamoeba histolytica; RNase III; AGO2;

D O I：

10.1016/j.exppara.2005.02.023

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Entamoeba histolytica, a protozoan parasite with variable DNA content and complex ploidity, has defied most efforts aimed at gene depletion using classical genetic methods. In this study, we identified and characterized two proteins involved in the RNA interference (RNAi) pathway, RNase III and AGO2. Our results strengthen the findings that an RNAi pathway does exist in this parasite. (c) 2005 Elsevier Inc. All rights reserved.

引用

页码：265 / 269

页数：5

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