Characterization and assessment of barnacle larval settlement-inducing activity of extracellular polymeric substances isolated from marine biofilm bacteria

被引：26

作者：

Siddik, Aboobucker ^{[1
]}

Satheesh, Sathianeson ^{[1
]}

机构：

[1] King Abdulaziz Univ, Fac Marine Sci, Dept Marine Biol, Jeddah, Saudi Arabia

来源：

SCIENTIFIC REPORTS | 2019年 / 9卷 / 1期

关键词：

POLYCHAETE HYDROIDS-ELEGANS; AMPHIBALANUS-AMPHITRITE; PSEUDOMONAS-AERUGINOSA; NEUROACTIVE COMPOUNDS; METAMORPHOSIS; INDUCTION; EXOPOLYSACCHARIDE; POLYSACCHARIDES; RELEVANCE; H-1-NMR;

D O I：

10.1038/s41598-019-54294-9

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Extracellular polymeric substances (EPSs) are the hydrated gelatinous matrix produced by microorganisms for attachment in a biofilm environment. In this study, the compositional variation between EPSs of three marine biofilm bacteria (Pseudoalteromonas shioyasakiensis, Vibrio harveyi and Planomicrobium sp.) were analysed by GC-MS, H-1 NMR, FT-IR and XRD and SEM. The ecological significance of exopolymers was assessed in vivo using marine model organism barnacle larvae for their settlement-inducing activity. Chemical analysis revealed the presence of glycan fucosylated oligosaccharides, tetraose, trisaccharides, iso-B-Pentasaccharides, sialyllactose, oligomannose, galacto-N-biose, difucosyl-para-lacto-N-neohexaose, 3'-sialyl N-acetyllactosamine and isoglobotriaose-beta-N(Acetyl)-Propargyl in all extracted EPSs. Bioassay results indicated that treatment of the barnacle larvae with EPSs from three bacterial strains enhanced settlement on substrates. In conclusion, this study highlighted the role of water-soluble EPSs in the invertebrate larval settlement on artificial materials.

引用

页数：15

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