The velvet Regulator VosA Governs Survival and Secondary Metabolism of Sexual Spores in Aspergillus nidulans

被引:16
|
作者
Kim, Min-Ju [1 ]
Lee, Mi-Kyung [2 ]
Pham, Huy Quang [3 ]
Gu, Myeong Ju [1 ]
Zhu, Bohan [4 ]
Son, Sung-Hun [1 ]
Hahn, Dongyup [1 ,5 ]
Shin, Jae-Ho [3 ,5 ]
Yu, Jae-Hyuk [6 ,7 ,8 ]
Park, Hee-Soo [1 ,5 ]
Han, Kap-Hoon [4 ]
机构
[1] Kyungpook Natl Univ, Sch Food Sci & Biotechnol, Daegu 41566, South Korea
[2] KRIBB, BRC, Jeongeup Si 56212, South Korea
[3] Kyungpook Natl Univ, Sch Appl Biosci, Daegu 41566, South Korea
[4] Woosuk Univ, Dept Pharmaceut Engn, Wonju 55338, South Korea
[5] Kyungpook Natl Univ, Dept Integrat Biol, Daegu 41566, South Korea
[6] Univ Wisconsin, Dept Bacteriol, Madison, WI 53706 USA
[7] Univ Wisconsin, Dept Genet, Madison, WI 53706 USA
[8] Konkuk Univ, Dept Syst Biotechnol, Seoul 05029, South Korea
基金
新加坡国家研究基金会;
关键词
VosA; sexual development; ascospores; Aspergillus nidulans; TRANSCRIPTION FACTOR; ASEXUAL SPORULATION; GENE; TREHALOSE; INSIGHTS; RESOURCE; FUNGI; NSDD;
D O I
10.3390/genes11010103
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
The velvet regulator VosA plays a pivotal role in asexual sporulation in the model filamentous fungus Aspergillus nidulans. In the present study, we characterize the roles of VosA in sexual spores (ascospores) in A. nidulans. During ascospore maturation, the deletion of vosA causes a rapid decrease in spore viability. The absence of vosA also results in a lack of trehalose biogenesis and decreased tolerance of ascospores to thermal and oxidative stresses. RNA-seq-based genome-wide expression analysis demonstrated that the loss of vosA leads to elevated expression of sterigmatocystin (ST) biosynthetic genes and a slight increase in ST production in ascospores. Moreover, the deletion of vosA causes upregulation of additional gene clusters associated with the biosynthesis of other secondary metabolites, including asperthecin, microperfuranone, and monodictyphenone. On the other hand, the lack of vosA results in the downregulation of various genes involved in primary metabolism. In addition, vosA deletion alters mRNA levels of genes associated with the cell wall integrity and trehalose biosynthesis. Overall, these results demonstrate that the velvet regulator VosA plays a key role in the maturation and the cellular and metabolic integrity of sexual spores in A. nidulans.
引用
收藏
页数:12
相关论文
共 50 条
  • [21] Homeobox proteins are essential for fungal differentiation and secondary metabolism in Aspergillus nidulans
    Son, Sung-Hun
    Son, Ye-Eun
    Cho, He-Jin
    Chen, Wanping
    Lee, Mi-Kyung
    Kim, Lee-Han
    Han, Dong-Min
    Park, Hee-Soo
    SCIENTIFIC REPORTS, 2020, 10 (01)
  • [22] Genetic engineering of Aspergillus nidulans secondary metabolism for "unnatural" natural products
    Liu, Ting
    Wang, Clay C. C.
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2013, 245
  • [23] A Bcl-2 Associated Athanogene (bagA) Modulates Sexual Development and Secondary Metabolism in the Filamentous Fungus Aspergillus nidulans
    Jain, Sachin
    Wiemann, Philipp
    Thill, Elizabeth
    Williams, Brett
    Keller, Nancy P.
    Kabbage, Mehdi
    FRONTIERS IN MICROBIOLOGY, 2018, 9
  • [24] The putative C2H2 transcription factor RocA is a novel regulator of development and secondary metabolism in Aspergillus nidulans
    Dong Chan Won
    Yong Jin Kim
    Da Hye Kim
    Hee-Moon Park
    Pil Jae Maeng
    Journal of Microbiology, 2020, 58 : 574 - 587
  • [25] Importin α is an essential nuclear import carrier adaptor required for proper sexual and asexual development and secondary metabolism in Aspergillus nidulans
    Araujo-Bazan, Lidia
    Dhingra, Sourabh
    Chu, John
    Fernandez-Martinez, Javier
    Calvo, Ana M.
    Espeso, Eduardo A.
    FUNGAL GENETICS AND BIOLOGY, 2009, 46 (6-7) : 506 - 515
  • [26] A Class 1 Histone Deacetylase as Major Regulator of Secondary Metabolite Production in Aspergillus nidulans
    Pidroni, Angelo
    Faber, Birgit
    Brosch, Gerald
    Bauer, Ingo
    Graessle, Stefan
    FRONTIERS IN MICROBIOLOGY, 2018, 9
  • [27] The Putative C2H2 Transcription Factor MtfA Is a Novel Regulator of Secondary Metabolism and Morphogenesis in Aspergillus nidulans
    Ramamoorthy, Vellaisamy
    Dhingra, Sourabh
    Kincaid, Alexander
    Shantappa, Sourabha
    Feng, Xuehuan
    Calvo, Ana M.
    PLOS ONE, 2013, 8 (09):
  • [28] The putative C2H2 transcription factor RocA is a novel regulator of development and secondary metabolism in Aspergillus nidulans
    Won, Dong Chan
    Kim, Yong Jin
    Kim, Hye
    Park, Hee-Moon
    Maeng, Pil Jae
    JOURNAL OF MICROBIOLOGY, 2020, 58 (07) : 574 - 587
  • [29] The sulfur metabolism regulator MetR is a global regulator controlling phytochrome-dependent light responses in Aspergillus nidulans
    Yu, Zhenzhong
    Gao, Jia
    Igbalajobi, Olumuyiwa
    Skoneczny, Marek
    Sienko, Marzena
    Maciejewska, Agnieszka M.
    Brzywczy, Jerzy
    Fischer, Reinhard
    SCIENCE BULLETIN, 2021, 66 (06) : 592 - 602
  • [30] THE SEXUAL AND ASEXUAL SPORES OF ASPERGILLUS-NIDULANS CONTAIN PARTIALLY OVERLAPPING SETS OF MESSENGER-RNAS
    JURGENSON, JE
    CHAMPE, SP
    EXPERIMENTAL MYCOLOGY, 1990, 14 (01): : 89 - 93