The velvet Regulator VosA Governs Survival and Secondary Metabolism of Sexual Spores in Aspergillus nidulans

被引:17
作者
Kim, Min-Ju [1 ]
Lee, Mi-Kyung [2 ]
Pham, Huy Quang [3 ]
Gu, Myeong Ju [1 ]
Zhu, Bohan [4 ]
Son, Sung-Hun [1 ]
Hahn, Dongyup [1 ,5 ]
Shin, Jae-Ho [3 ,5 ]
Yu, Jae-Hyuk [6 ,7 ,8 ]
Park, Hee-Soo [1 ,5 ]
Han, Kap-Hoon [4 ]
机构
[1] Kyungpook Natl Univ, Sch Food Sci & Biotechnol, Daegu 41566, South Korea
[2] KRIBB, BRC, Jeongeup Si 56212, South Korea
[3] Kyungpook Natl Univ, Sch Appl Biosci, Daegu 41566, South Korea
[4] Woosuk Univ, Dept Pharmaceut Engn, Wonju 55338, South Korea
[5] Kyungpook Natl Univ, Dept Integrat Biol, Daegu 41566, South Korea
[6] Univ Wisconsin, Dept Bacteriol, Madison, WI 53706 USA
[7] Univ Wisconsin, Dept Genet, Madison, WI 53706 USA
[8] Konkuk Univ, Dept Syst Biotechnol, Seoul 05029, South Korea
基金
新加坡国家研究基金会;
关键词
VosA; sexual development; ascospores; Aspergillus nidulans; TRANSCRIPTION FACTOR; ASEXUAL SPORULATION; GENE; TREHALOSE; INSIGHTS; RESOURCE; FUNGI; NSDD;
D O I
10.3390/genes11010103
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
The velvet regulator VosA plays a pivotal role in asexual sporulation in the model filamentous fungus Aspergillus nidulans. In the present study, we characterize the roles of VosA in sexual spores (ascospores) in A. nidulans. During ascospore maturation, the deletion of vosA causes a rapid decrease in spore viability. The absence of vosA also results in a lack of trehalose biogenesis and decreased tolerance of ascospores to thermal and oxidative stresses. RNA-seq-based genome-wide expression analysis demonstrated that the loss of vosA leads to elevated expression of sterigmatocystin (ST) biosynthetic genes and a slight increase in ST production in ascospores. Moreover, the deletion of vosA causes upregulation of additional gene clusters associated with the biosynthesis of other secondary metabolites, including asperthecin, microperfuranone, and monodictyphenone. On the other hand, the lack of vosA results in the downregulation of various genes involved in primary metabolism. In addition, vosA deletion alters mRNA levels of genes associated with the cell wall integrity and trehalose biosynthesis. Overall, these results demonstrate that the velvet regulator VosA plays a key role in the maturation and the cellular and metabolic integrity of sexual spores in A. nidulans.
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页数:12
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