Separation of minor coumarins from Peucedanum praeruptorum using HSCCC and preparative HPLC guided by HPLC/MS

被引：46

作者：

Hou, Zhiguo ^{[1
]}

Luo, Jianguang ^{[1
]}

Wang, Junsong ^{[1
]}

Kong, Lingyi ^{[1
]}

机构：

[1] China Pharmaceut Univ, Dept Nat Med Chem, Nanjing 210009, Peoples R China

来源：

SEPARATION AND PURIFICATION TECHNOLOGY | 2010年 / 75卷 / 02期

基金：

中国国家自然科学基金;

关键词：

HSCCC; Prep-HPLC; HPLC/MS; Qianhucoumarin J; Peucedanum praeruptorum Dunn; CHROMATOGRAPHY; CONSTITUENTS;

D O I：

10.1016/j.seppur.2010.08.007

中图分类号：

TQ [化学工业];

学科分类号：

0817 ;

摘要：

Two minor coumarins, including a new compound qianhucoumarin J, were isolated from Peucedanum praeruptorum by a multi-step separation procedure using high-speed counter-current chromatography (HSCCC) and preparative high performance liquid chromatography (prep-HPLC) monitored by high performance liquid chromatography coupled with mass spectrometry (HPLC/MS) analysis. The crude extract was analyzed first by HPLC/MS to detect unknown compounds. The target compounds were enriched by HSCCC, using a two-phase solvent system of petroleum ether-ethyl acetate-methanol-water (5:5:6:4, v/v) and then isolated and purified by preparative HPLC monitored by HPLC/MS. The structure of the new compound was elucidated mainly by analysis of its 1D and 2D NMR spectral data. Its absolute configuration was determined by chemical degradation followed by chiral HPLC analysis. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：132 / 137

页数：6

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