Prenatal choline supplementation improves biomarkers of maternal docosahexaenoic acid (DHA) status among pregnant participants consuming supplemental DHA: a randomized controlled trial

被引：10

作者：

Klatt, Kevin C. ^{[1
,2
,3
]}

McDougall, Melissa Q. ^{[1
]}

Malysheva, Olga, V ^{[1
]}

Taesuwan, Siraphat ^{[1
,4
]}

Loinard-Gonzalez, Aura P. ^{[1
]}

Nevins, Julie E. H. ^{[1
]}

Beckman, Kara ^{[1
]}

Bhawal, Ruchika ^{[5
]}

Anderson, Elizabeth ^{[5
]}

Zhang, Sheng ^{[5
]}

Bender, Erica ^{[1
]}

Jackson, Kristina H. ^{[6
]}

King, D. Janette ^{[7
]}

Dyer, Roger A. ^{[7
]}

Devapatla, Srisatish ^{[8
]}

Vidavalur, Ramesh ^{[8
]}

Brenna, J. Thomas ^{[9
]}

Caudill, Marie A. ^{[1
]}

机构：

[1] Cornell Univ, Div Nutr Sci, Ithaca, NY 14853 USA

[2] Baylor Coll Med, Childrens Nutr Res Ctr, Ctr Precis Environm Hlth, Houston, TX 77030 USA

[3] Univ Calif Berkeley, Nutr Sci & Toxicol, Berkeley, CA 94720 USA

[4] Chiang Mai Univ, Fac Agroind, Chiang Mai, Thailand

[5] Cornell Univ, Prote & Metabol Facil, Ithaca, NY USA

[6] OmegaQuant Analyt LLC, Sioux Falls, SD USA

[7] Univ British Columbia, BC Childrens Hosp, Analyt Core Metabol & Nutr, Res Inst, Vancouver, BC, Canada

[8] Cayuga Med Ctr, Ithaca, NY USA

[9] Univ Texas Austin, Dept Pediat, Austin, TX 78712 USA

来源：

AMERICAN JOURNAL OF CLINICAL NUTRITION | 2022年 / 116卷 / 03期

关键词：

prenatal choline supplementation; pregnancy; docosahexaenoic acid; PEMT pathway; omega-3 polyunsaturated fatty acids; stable isotope; N-METHYLTRANSFERASE ACTIVITY; POLYUNSATURATED FATTY-ACIDS; METABOLISM; PLASMA; PHOSPHATIDYLCHOLINE; EXTRACTION; WOMEN;

D O I：

10.1093/ajcn/nqac147

中图分类号：

R15 [营养卫生、食品卫生]; TS201 [基础科学];

学科分类号：

100403 ;

摘要：

Background: Dietary methyl donors (e.g., choline) support the activity of the phosphatidylethanolamine N-methyltransferase (PEMT) pathway, which generates phosphatidylcholine (PC) molecules enriched in DHA that are exported from the liver and made available to extrahepatic tissues. Objectives: This study investigated the effect of prenatal choline supplementation on biomarkers of DHA status among pregnant participants consuming supplemental DHA. Methods: Pregnant participants (n = 30) were randomly assigned to receive supplemental choline intakes of 550 mg/d [500 mg/d d0-choline + 50 mg/d deuterium-labeled choline (d9-choline); intervention] or 25 mg/d (25 mg/d d9-choline; control) from gestational week (GW) 12-16 until delivery. All participants received a daily 200-mg DHA supplement and consumed self-selected diets. Fasting blood samples were obtained at baseline, GW 20-24, and GW 28-32; maternal/cord blood was obtained at delivery. Mixed-effects linear models were used to assess the impact of prenatal choline supplementation on maternal and newborn DHA status. Results: Choline supplementation (550 vs. 25 mg/d) did not achieve a statistically significant intervention x time interaction for RBC PC-DMA (P = 0.11); a significant interaction was observed for plasma PC-DI IA and RBC total DHA, with choline supplementation yielding higher levels (+32-38% and +8-11%, respectively) at GW 28-32 (P < 0.05) and delivery (P < 0.005). A main effect of choline supplementation on plasma total DHA was also observed (P = 0.018); its interaction with time was not significant (P = 0.068). Compared with controls, the intervention group exhibited higher (P = 0.007; main effect) plasma enrichment of d3-PC (d3-PC/total PC). Moreover, the ratio of d3-PC to d9-PC was higher (+50-67%; P < 0.001) in the choline intervention arm (vs. control) at GW 20-24, GW 28-32, and delivery. Conclusions: Prenatal choline supplementation improves hepatic DHA export and biomarkers of DHA status by bolstering methyl group supply for PEMT activity among pregnant participants consuming supplemental DHA.

引用

页码：820 / 832

页数：13

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