Point-of-care-testing of nucleic acids by microfluidics

被引：140

作者：

Zhang, Lu ^{[1
]}

Ding, Baozhi ^{[3
]}

Chen, Qinghua ^{[2
]}

Feng, Qiang ^{[2
]}

Lin, Ling ^{[2
]}

Sun, Jiashu ^{[2
]}

机构：

[1] Capital Normal Univ, Dept Chem, Beijing 100048, Peoples R China

[2] Natl Ctr Nanosci & Technol, CAS Ctr Excellence Nanosci, CAS Key Lab Standardizat & Measurement Nanotechno, Beijing 100190, Peoples R China

[3] Shanghai Jiao Tong Univ, Shanghai Peoples Hosp 9, Dept Orthopaed Surg, Shanghai Key Lab Orthopaed Implants,Sch Med, Shanghai 200011, Peoples R China

来源：

TRAC-TRENDS IN ANALYTICAL CHEMISTRY | 2017年 / 94卷

关键词：

Point-of-care testing (POCT); Nucleic acid; Microfluidics; Diagnostics; Integration; MEDIATED ISOTHERMAL AMPLIFICATION; VISUAL QUANTITATIVE DETECTION; REAL-TIME PCR; ON-A-CHIP; MOLECULAR DIAGNOSIS; LABEL-FREE; LOW-COST; SAMPLE PREPARATION; DNA AMPLIFICATION; INSTRUMENT-FREE;

D O I：

10.1016/j.trac.2017.07.013

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Nucleic acid detection offers critical information in diagnosing pathogen infection and monitoring disease treatment. Point-of-care testing (POCT), with ideal characteristics such as rapidness, user-friendly, sensitivity, specificity, and robustness, has drawn wide attention, especially in resource-poor settings where lack the healthcare infrastructure. Accompanied by the development of microfluidic technologies and new amplification methods, POCT of nucleic acids greatly improves health monitoring and prevents outbreak of infectious diseases. This review summarizes recent advances in diagnostics of nucleic acids in POC formats, and discusses challenges associated with POCT of nucleic acids. (C) 2017 Elsevier B.V. All rights reserved.

引用

页码：106 / 116

页数：11

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