Cantharidin blisters: a technique for investigating leukocyte trafficking and cytokine production at sites of inflammation in humans

被引:58
作者
Day, RM
Harbord, M
Forbes, A
Segal, AW
机构
[1] St Marks Hosp, St Marks Hosp & Acad Inst, Harrow HA1 3UJ, Middx, England
[2] UCL, Rayne Inst, Ctr Mol Med, London WC1E 6JJ, England
基金
英国惠康基金;
关键词
inflammation; leukocytes; chemokines; cytokines; cantharidin;
D O I
10.1016/S0022-1759(01)00467-7
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A skin blister technique is described which allows the investigation of acute inflammation in humans in vivo. Filter paper discs are placed on the skin, impregnated with cantharidin and covered with impermeable film held by adhesive tape. The assembly is easily applied, unobtrusive, stable and may be worn during normal activities. The blister formed at 24 h contains approximately 5 X 10(5)-5 X 10(6) cells, predominantly neutrophils and macrophages. Inflammatory cytokines and chemotactic factors are detectable in the blister fluid. The technique is useful for characterizing the acute inflammatory response in health and disease. (C) 2001 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:213 / 220
页数:8
相关论文
共 19 条
[1]   CANTHARIDE ACANTHOLYSIS - ENDOGENOUS PROTEASE ACTIVATION LEADING TO DESMOSOMAL PLAQUE DISSOLUTION [J].
BERTAUX, B ;
PROST, C ;
HESLAN, M ;
DUBERTRET, L .
BRITISH JOURNAL OF DERMATOLOGY, 1988, 118 (02) :157-165
[2]   Direct assessment of peripheral pharmacokinetics in humans:: comparison between cantharides blister fluid sampling, in vivo microdialysis and saliva sampling [J].
Brunner, M ;
Schmiedberger, A ;
Schmid, R ;
Jäger, D ;
Piegler, E ;
Eichler, HG ;
Müller, M .
BRITISH JOURNAL OF CLINICAL PHARMACOLOGY, 1998, 46 (05) :425-431
[3]   Leukocyte L-selectin is up-regulated after mechanical trauma in adults [J].
Cocks, RA ;
Chan, TYF ;
Rainer, TH .
JOURNAL OF TRAUMA-INJURY INFECTION AND CRITICAL CARE, 1998, 45 (01) :1-6
[4]   On the edge: the physiological and pathophysiological role of chemokines during inflammatory and immunological responses [J].
DeVries, ME ;
Ran, L ;
Kelvin, DJ .
SEMINARS IN IMMUNOLOGY, 1999, 11 (02) :95-104
[5]   LYMPHANGITIS CAUSED BY CANTHARIDIN [J].
DILAIMY, M .
ARCHIVES OF DERMATOLOGY, 1975, 111 (08) :1073-1073
[6]   A 48-WELL MICRO CHEMOTAXIS ASSEMBLY FOR RAPID AND ACCURATE MEASUREMENT OF LEUKOCYTE MIGRATION [J].
FALK, W ;
GOODWIN, RH ;
LEONARD, EJ .
JOURNAL OF IMMUNOLOGICAL METHODS, 1980, 33 (03) :239-247
[7]   CANTHARIDIN, ANOTHER NATURAL TOXIN THAT INHIBITS THE ACTIVITY OF SERINE THREONINE PROTEIN PHOSPHATASES TYPE-1 AND TYPE-2A [J].
HONKANEN, RE .
FEBS LETTERS, 1993, 330 (03) :283-286
[9]  
KIISTALA U, 1964, LANCET, V1, P1444
[10]  
MASS MF, 1975, J LAB CLIN MED, V86, P1040