Label-free Raman observation of cytochrome c dynamics during apoptosis

被引：387

作者：

Okada, Masaya ^{[1
]}

Smith, Nicholas Isaac ^{[2
]}

Palonpon, Almar Flotildes ^{[3
]}

Endo, Hiromi ^{[1
]}

Kawata, Satoshi ^{[1
,4
]}

Sodeoka, Mikiko ^{[3
,4
]}

Fujita, Katsumasa ^{[1
,3
]}

机构：

[1] Osaka Univ, Dept Appl Phys, Suita, Osaka 5650871, Japan

[2] Osaka Univ, Immunol Frontier Res Ctr, Suita, Osaka 5650871, Japan

[3] Japan Sci & Technol Agcy, Sodeoka Live Cell Chem Project, Wako, Saitama 3510198, Japan

[4] RIKEN, Adv Sci Inst, Wako, Saitama 3510198, Japan

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2012年 / 109卷 / 01期

基金：

日本科学技术振兴机构;

关键词：

label-free imaging; protein signaling; Raman microscopy; redox state; CELLS; SPECTROSCOPY; MICROSCOPY; SCATTERING; SPECTRA; PROTEIN; RELEASE; PROBE;

D O I：

10.1073/pnas.1107524108

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We performed label-free observation of molecular dynamics in apoptotic cells by Raman microscopy. Dynamic changes in cytochrome c distribution at the Raman band of 750 cm(-1) were observed after adding an apoptosis inducer to the cells. The comparison of mitochondria fluorescence images and Raman images of cytochrome c confirmed that changes in cytochrome c distribution can be distinguished as release of cytochrome c from mitochondria. Our observation also revealed that the redox state of cytochrome c was maintained during the release from the mitochondria. Monitoring mitochondrial membrane potential with JC-1 dye confirmed that the observed cytochrome c release was associated with apoptosis.

引用

页码：28 / 32

页数：5

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