Micropropagation of Salvia wagneriana Polak and hairy root cultures with rosmarinic acid production

被引:9
作者
Ruffoni, Barbara [1 ]
Bertoli, Alessandra [2 ,4 ]
Pistelli, Laura [3 ,4 ]
Pistelli, Luisa [2 ,4 ]
机构
[1] Consiglio Ric Agr & Anal Econ Agr CREA FSO, Unita Ric Floricoltura & Specie Ornamentali Corso, San Remo, Italy
[2] Univ Pisa, Dipartimento Farm, Pisa, Italy
[3] Univ Pisa, Dipartimento Sci Agr Alimentari & Agroambientali, Pisa, Italy
[4] Univ Pisa, Ctr Interdipartimentale Ric Nutraceut & Alimentaz, Pisa, Italy
关键词
Salvia wagneriana Polak; Agrobacterium rhizogenes; elicitation; casein hydrolysate; LC-ESI-DAD-MS; rosmarinic acid; CELL-SUSPENSION CULTURES; METHYL JASMONATE; MILTIORRHIZA; ELICITATION; OFFICINALIS; GROWTH; L; ACCUMULATION;
D O I
10.1080/14786419.2015.1120725
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
Salvia wagneriana Polak is a tropical species native to Central America, well adapted to grow in the Mediterranean basin for garden decoration. Micropropagation has been assessed from axillary shoots of adult plants using a Murashige and Skoog basal medium, with the addition of 1.33-M 6-benzylaminopurine for shoot proliferation; the subsequent rooting phase occurred in plant growth regulator-free medium. The plants were successfully acclimatised with high survival frequency. Hairy roots were induced after co-cultivation of leaf lamina and petiole fragments with Agrobacterium rhizogenes and confirmed by PCR. The establishment and proliferation of the selected HRD3 line were obtained in hormone-free liquid medium and the production of rosmarinic acid (RA) was evaluated after elicitation. The analysis of RA was performed by LC-ESI-DAD-MS in the hydroalcoholic extracts. The addition of casein hydrolysate increased the RA production, whereas no enrichment was observed after the elicitation with jasmonic acid.
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页码:2538 / 2544
页数:7
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