RNA fluorescence in situ hybridization using 3-cyanovinylcarbazole modified oligodeoxyribonucleotides as photo-cross-linkable probes

被引：15

作者：

Fujimoto, Kenzo ^{[1
]}

Toyosato, Kei ^{[1
]}

Nakamura, Shigetaka ^{[1
]}

Sakamoto, Takashi ^{[1
]}

机构：

[1] Japan Adv Inst Sci & Technol, Sch Mat Sci, 1-1 Asahi Dai, Nomi, Ishikawa 9231292, Japan

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2016年 / 26卷 / 21期

关键词：

Photo-cross-link; 3-Cyanovinylcarbazole nucleoside; Fluorescence in situ hybridization; 16S rRNA; LINKING REACTION; MOLECULAR BEACON; CY3;

D O I：

10.1016/j.bmcl.2016.09.035

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Photo-cross-linkable fluorescent oligodeoxyribonucleotides having 3-cyanovinylcarbazole nucleoside were applied to fluorescence in situ hybridization (FISH) based 16S rRNA detection in Escherichia coli cells. As the photo-cross-linked probe/rRNA hybrid was stable under the denaturing condition, decrease of the fluorescence signal through the washing process was prevented. The thermally irreversible hybridization property also enabled stable hybridization with the structured region on the target RNA, and facilitated design of the sequence for the FISH probe. Further development of the method might contribute to quantitative and stable FISH staining. (C) 2016 Elsevier Ltd. All rights reserved.

引用

页码：5312 / 5314

页数：3

共 16 条

[1] Application of locked nucleic acid-based probes in fluorescence in situ hybridization [J].

Fontenete, Silvia ;

Carvalho, Daniel ;

Guimares, Nuno ;

Madureira, Pedro ;

Figueiredo, Ceu ;

Wengel, Jesper ;

Azevedo, Nuno Filipe .

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2016, 100 (13) :5897-5906

[2]

Fuchs BM, 1998, APPL ENVIRON MICROB, V64, P4973

[3] Details of the Ultrafast DNA Photo-Cross-Linking Reaction of 3-Cyanovinylcarbazole Nucleoside: Cis-Trans Isomeric Effect and the Application for SNP-Based Genotyping [J].

Fujimoto, Kenzo ;

Yamada, Asuka ;

Yoshimura, Yoshinaga ;

Tsukaguch, Tadashi ;

Sakamoto, Takashi .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2013, 135 (43) :16161-16167

[4] LESSONS FROM AN EVOLVING RIBOSOMAL-RNA - 16S AND 23S RIBOSOMAL-RNA STRUCTURES FROM A COMPARATIVE PERSPECTIVE [J].

GUTELL, RR ;

LARSEN, N ;

WOESE, CR .

MICROBIOLOGICAL REVIEWS, 1994, 58 (01) :10-26

[5] LNA-enhanced DNA FIT-probes for multicolour RNA imaging [J].

Hoevelmann, F. ;

Gaspar, I. ;

Chamiolo, J. ;

Kasper, M. ;

Steffen, J. ;

Ephrussi, A. ;

Seitz, O. .

CHEMICAL SCIENCE, 2016, 7 (01) :128-135

[6] METAPHASE AND INTERPHASE FLUORESCENCE IN-SITU HYBRIDIZATION MAPPING OF THE RICE GENOME WITH BACTERIAL ARTIFICIAL CHROMOSOMES [J].

JIANG, JM ;

GILL, BS ;

WANG, GL ;

RONALD, PC ;

WARD, DC .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (10) :4487-4491

[7] A stem-less probe using spontaneous pairing between Cy3 and quencher for RNA detection [J].

Kashida, Hiromu ;

Morimoto, Kazuhiro ;

Asanuma, Hiroyuki .

SCIENCE AND TECHNOLOGY OF ADVANCED MATERIALS, 2016, 17 (01) :267-273

[8] Molecular design of Cy3 derivative for highly sensitive in-stem molecular beacon and its application to the wash-free FISH [J].

Kashida, Hiromu ;

Osawa, Takuya ;

Morimoto, Kazuhiro ;

Kamiya, Yukiko ;

Asanuma, Hiroyuki .

BIOORGANIC & MEDICINAL CHEMISTRY, 2015, 23 (08) :1758-1762

[9] Sensitive quantitative detection of commensal bacteria by rRNA-Targeted reverse Transcription-PCR (vol 73, pg 32, 2007) [J].

Matsuda, Kazunori ;

Tsuji, Hirokazu ;

Asahara, Takashi ;

Kado, Yukiko ;

Nomoto, Koji .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2007, 73 (20) :6695-6695

[10] Ultrasensitive Molecular Beacon Designed with Totally Serinol Nucleic Acid (SNA) for Monitoring mRNA in Cells [J].

Murayama, Keiji ;

Kamiya, Yukiko ;

Kashida, Hiromu ;

Asanuma, Hiroyuki .

CHEMBIOCHEM, 2015, 16 (09) :1298-1301

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