Light sheet microscopy of living or cleared specimens

被引：124

作者：

Keller, Philipp J. ^{[1
]}

Dodt, Hans-Ulrich ^{[2
]}

机构：

[1] Howard Hughes Med Inst, Ashburn, VA 20147 USA

[2] Vienna Univ Technol, Inst Solid State Elect, Dept Bioelect, A-1040 Vienna, Austria

来源：

CURRENT OPINION IN NEUROBIOLOGY | 2012年 / 22卷 / 01期

关键词：

PLANE ILLUMINATION MICROSCOPY; RECONSTRUCTION; DEEP;

D O I：

10.1016/j.conb.2011.08.003

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Light sheet microscopy is a versatile imaging technique with a unique combination of capabilities. It provides high imaging speed, high signal-to-noise ratio and low levels of photobleaching and phototoxic effects. These properties are crucial in a wide range of applications in the life sciences, from live imaging of fast dynamic processes in single cells to long-term observation of developmental dynamics in entire large organisms. When combined with tissue clearing methods, light sheet microscopy furthermore allows rapid imaging of large specimens with excellent coverage and high spatial resolution. Even samples up to the size of entire mammalian brains can be efficiently recorded and quantitatively analyzed. Here, we provide an overview of the history of light sheet microscopy, review the development of tissue clearing methods, and discuss recent technical breakthroughs that have the potential to influence the future direction of the field.

引用

页码：138 / 143

页数：6

共 47 条

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