Transportation and storage of cryosections in PBS-Dulbecco

被引:0
作者
Eneström, S
Nilsson, A
Peen, E
机构
[1] Linkoping Univ Hosp, Div Mol & Immunol Pathol, S-58185 Linkoping, Sweden
[2] Univ Bergen, Haukeland Hosp, Dept Internal Med B, N-5021 Bergen, Norway
关键词
cryosections; storage; transportation;
D O I
10.3109/10520299909066475
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Tissue pieces that cannot be fixed and embedded before immunostaining must be cryosectioned and stained quickly. We describe an easy and reliable technique to keep cryosections with immune complexes and complement stored for several weeks until the final immunostaining, The same technique was used for long distance transportation of cryosections and it was extensively tested for human kidney biopsies with excellent results.
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页码:34 / 39
页数:6
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