Serum phytosterols associate with T helper 1 cytokine concentration in pregnant women

The dietary phytosterols have been demonstrated to modulate CD4(+)T-cell polarization in cells, animals, and humans. However, T helper (Th)1/Th2 dichotomy has rarely been correlated with phytosterols during pregnancy. The present study investigated associations between the serum cytokines and serum phytosterols in 100 pregnant women at 34- to 37-week gestation and their offspring. The results showed that serum concentrations of interferon (IFN)-gamma, tumor necrosis factor (TNF)-alpha, and total Th1 cytokines were positively associated with serum beta-sitosterol level, adjusting for age, BMI, and serum cholesterol. Serum IFN-gamma and total Th1 cytokine concentrations positively correlated with total phytosterol concentration, controlling age, BMI, and serum cholesterol. Moreover, none of the cytokines measured correlated with phytosterol concentration in the newborns. Our results show that serum Th1 cytokine concentrations, but not Th2 levels, are positively associated with serum phytosterols in pregnant women. These findings implicate that phytosterols modulate Th1/Th2 balance by inducing Th1 secretions in pregnant women.