Worker Honeybee Brain Proteome

被引：35

作者：

Hernandez, Liudy G. ^{[2
,3
]}

Lu, Bingwen ^{[4
]}

da Cruz, Gabriel C. N. ^{[3
]}

Calabria, Luciana K. ^{[5
]}

Martins, Natalia F. ^{[6
]}

Togawa, Roberto ^{[6
]}

Espindola, Foued S. ^{[5
]}

Yates, John R., III ^{[4
]}

Cunha, Ricardo B. ^{[1
]}

de Sousa, Marcelo V. ^{[3
]}

机构：

[1] Univ Brasilia, Lab Bioanalyt Chem, Inst Chem, Brasilia, DF, Brazil

[2] CEADEN, Mass Spectrometry Grp, Dept Phys, Havana, Cuba

[3] Univ Brasilia, Brazilian Ctr Prot Res, Dept Cell Biol, Brasilia, DF, Brazil

[4] Scripps Res Inst, Dept Physiol Chem, La Jolla, CA 92037 USA

[5] Univ Fed Uberlandia, Genet & Biochem Inst, BR-38400 Uberlandia, MG, Brazil

[6] Embrapa Genet Resources & Biotechnol, Bioinformat Lab, Brasilia, DF, Brazil

来源：

JOURNAL OF PROTEOME RESEARCH | 2012年 / 11卷 / 03期

基金：

美国国家卫生研究院;

关键词：

Apis mellifera; brain proteome; differential expression; honeybee subcastes; MudPIT; APIS-MELLIFERA WORKERS; DIVISION-OF-LABOR; HYPOPHARYNGEAL-GLAND; GENE-EXPRESSION; MUSHROOM BODIES; CA2+-BINDING PROTEIN; JUVENILE-HORMONE; BEE; BEHAVIOR; AGE;

D O I：

10.1021/pr2007818

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A large-scale mapping of the worker honeybee brain proteome was achieved by MudPIT. We identified 2742 proteins from forager and nurse honeybee brain samples; 17% of the total proteins were found to be differentially expressed by spectral count sampling statistics and a G-test. Sequences were compared with the EuKaryotic Orthologous Groups (KOG) catalog set using BLASTX and then categorized into the major KOG categories of most similar sequences. According to this categorization, nurse brain showed increased expression of proteins implicated in translation, ribosomal structure, and biogenesis (14.5%) compared with forager (1.8%). Experienced foragers overexpressed proteins involved in energy production and conversion, showing an extensive difference in this set of proteins (17%) in relation to the nurse subcaste (0.6%). Examples of proteins selectively expressed in each subcaste were analyzed. A comparison between these MudPIT experiments and previous 2-DE experiments revealed nine coincident proteins differentially expressed in both methodologies.

引用

页码：1485 / 1493

页数：9

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