Composition gradient static light scattering: A new technique for rapid detection and quantitative characterization of reversible macromolecular hetero-associations in solution

被引：38

作者：

Attri, AK

Minton, AP ^{[1
]}

机构：

[1] NIDDKD, Lab Biochem & Genet, NIH, US Dept HHS, Bethesda, MD 20892 USA

[2] Jawaharlal Nehru Univ, Sch Environm Sci, New Delhi 110067, India

来源：

ANALYTICAL BIOCHEMISTRY | 2005年 / 346卷 / 01期

关键词：

protein-protein association equilibria; Rayleigh light scattering;

D O I：

10.1016/j.ab.2005.08.013

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method for rapid detection and quantitative characterization of associations between two proteins in solution is presented, based upon continuous measurement of the Rayleigh light scattering and UV absorbance of a solution whose composition is varied with time in a controlled fashion. Collection and subsequent analysis of data are both rapid and semiautomatic. The results of validation experiments demonstrate that the technique can correctly identify complexes and reliably evaluate equilibrium constants for hetero-association. (C) 2005 Elsevier Inc. All rights reserved.

引用

页码：132 / 138

页数：7

共 13 条

[1] New methods for measuring macromolecular interactions in solution via static light scattering: basic methodolog, and application to nonassociating and self-associating proteins [J].