DescePrep Significantly Increases Descemet Membrane Endothelial Keratoplasty Processing Efficiency and Success Rate in Diabetic Human Donor Corneas in Comparison With Manual Dissection

被引：1

作者：

Solley, Katherine D. ^{[1
]}

Berges, Alexandra J. ^{[3
]}

Phillips, Elizabeth ^{[1
]}

Ding, Andy S. ^{[3
]}

Diaz, Christine M. ^{[1
]}

Pramanik, Sudeep ^{[1
,4
]}

Yazdi, Youseph ^{[2
,5
]}

Parikh, Kunal S. ^{[1
,2
,5
,6
,7
]}

机构：

[1] Eyedea Med, Res & Dev, Baltimore, MD USA

[2] Johns Hopkins Univ, Ctr Bioengn Innovat & Design, Baltimore, MD USA

[3] Johns Hopkins Univ, Sch Med, Baltimore, MD USA

[4] Midatlant Cornea Consultants, Baltimore, MD USA

[5] Johns Hopkins Univ, Sch Med, Dept Biomed Engn, Baltimore, MD 21205 USA

[6] Johns Hopkins Univ, Sch Med, Ctr Nanomed, Baltimore, MD USA

[7] Johns Hopkins Univ, Sch Med, Wilmer Eye Inst, Dept Ophthalmol, Baltimore, MD 21205 USA

来源：

CORNEA | 2022年 / 41卷 / 10期

基金：

美国国家科学基金会;

关键词：

Descemet membrane endothelial keratoplasty; corneal transplantation; eye banking; graft; Fuchs dystrophy; TISSUE-PREPARATION; GRAFT PREPARATION; MELLITUS; IMPACT; RISK;

D O I：

10.1097/ICO.0000000000003096

中图分类号：

R77 [眼科学];

学科分类号：

100212 ;

摘要：

Purpose: The purpose of this study was to compare the safety, efficacy, and efficiency of a Descemet membrane endothelial keratoplasty (DMEK) graft processing device, DescePrep, with a manual dissection technique through the measurement of tissue yield, processing time, and graft viability in nondiabetic and diabetic donor corneas. Methods: Nondiabetic (n = 20) and diabetic (n = 20) donor corneas were processed using DescePrep, which standardizes the liquid bubble technique. Nondiabetic (n = 20) and diabetic (n = 24) donor corneas were also processed through manual dissection. Corneas were stained, processed, and then evaluated for processing success rate and time. Randomly selected corneas (n = 5, each) were evaluated for cell viability using live/dead staining. Results: One hundred percent of nondiabetic and 95% of diabetic corneas were processed successfully with DescePrep in an average of 3.37 minutes. Ninety percent of nondiabetic and 50% of diabetic corneas were processed successfully with manual dissection in an average of 9.87 minutes. DescePrep had a significantly lower processing time (P < 0.0001) and significantly higher success rate in comparison with manual dissection. DescePrep grafts had an average cell viability of 91.1% +/- 3.3% in nondiabetic and 91.5% +/- 2.4% in diabetic corneas. Grafts prepared with manual dissection had an average cell viability of 89.5% +/- 5.8% in nondiabetic and 88.1% +/- 4.3% in diabetic corneas. Conclusions: DescePrep provides a more effective and efficient method of cornea preparation in comparison with the current standard, particularly in diabetic corneas, while providing comparable cell viability. Thus, DescePrep offers standardized DMEK processing that produces high-quality grafts at high yields, with the potential to expand access and improve the quality of DMEK graft preparation in a larger pool of donors.

引用

页码：1284 / 1290

页数：7

共 19 条

[1] Global cell-by-cell evaluation of endothelial viability after two methods of graft preparation in Descemet membrane endothelial keratoplasty [J].