Strain typing among enterococci isolated from home-made Pecorino Sardo cheese

被引：3

作者：

Mannu, L ^{[1
]}

Paba, A

Pes, M

Floris, R

Scintu, MF

Morelli, L

机构：

[1] Ist Zootecn & Caseario Sardegna, I-07040 Olmedo, Italy

[2] Univ Cattolica Sacro Cuore, Ist Microbiol, I-29100 Piacenza, Italy

来源：

FEMS MICROBIOLOGY LETTERS | 1999年 / 170卷 / 01期

关键词：

strain typing; Enterococcus; RAPD-PCR; plasmid profile; pulsed-field gel electrophoresis;

D O I：

10.1111/j.1574-6968.1999.tb13351.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Three molecular techniques (RAPD-polymerase chain reaction analysis, plasmid profile and pulsed-field gel electrophoresis) were used for a preliminary approach to type, at strain level, enterococci isolated from a 24-h-old home-made Pecorino Sardo (protected designation of origin) cheese. A high genetic polymorphism was found. Clusters obtained by the RAPD technique and plasmid profile analysis contained different strains. Pulsed-field gel electrophoresis proved to be an efficient and highly reproducible typing method. In addition, by combining the results from plasmid profile analysis and pulsed-field gel electrophoresis, it was possible to identify closely related strains probably belonging to the same clonal lineage. (C) 1999 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：25 / 30

页数：6

共 18 条

[1] Comparison of three methods for the molecular typing of Singapore isolates of enterococci with high-level aminoglycoside resistances [J].