Persulfidation proteome reveals the regulation of protein function by hydrogen sulfide in diverse biological processes in Arabidopsis

被引:240
作者
Aroca, Angeles [1 ]
Benito, Juan M. [2 ,3 ]
Gotor, Cecilia [1 ]
Romero, Luis C. [1 ]
机构
[1] CSIC, Inst Bioquim Vegetal & Fotosintesis, Ave Amer Vespucio,49, Seville 41092, Spain
[2] CSIC, Inst Invest Quim, Ave Amer Vespucio,49, Seville 41092, Spain
[3] Univ Seville, Ave Amer Vespucio,49, Seville 41092, Spain
关键词
Cysteine; hydrogen sulfide; mass spectrometry; persulfidation; post-translational modification; proteomics; TAG-SWITCH METHOD; S-SULFHYDRATION; ABSCISIC-ACID; NITRIC-OXIDE; GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE; CYSTEINE HOMEOSTASIS; SIGNALING PATHWAYS; OXIDATIVE STRESS; H2S; METABOLISM;
D O I
10.1093/jxb/erx294
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Hydrogen sulfide-mediated signaling pathways regulate many physiological and pathophysiological processes in mammalian and plant systems. The molecular mechanism by which hydrogen sulfide exerts its action involves the post-translational modification of cysteine residues to form a persulfidated thiol motif, a process called protein persulfidation. We have developed a comparative and quantitative proteomic analysis approach for the detection of endogenous persulfidated proteins in wild-type Arabidopsis and L-CYSTEINE DESULFHYDRASE 1 mutant leaves using the tag-switch method. The 2015 identified persulfidated proteins were isolated from plants grown under controlled conditions, and therefore, at least 5% of the entire Arabidopsis proteome may undergo persulfidation under baseline conditions. Bioinformatic analysis revealed that persulfidated cysteines participate in a wide range of biological functions, regulating important processes such as carbon metabolism, plant responses to abiotic and biotic stresses, plant growth and development, and RNA translation. Quantitative analysis in both genetic backgrounds reveals that protein persulfidation is mainly involved in primary metabolic pathways such as the tricarboxylic acid cycle, glycolysis, and the Calvin cycle, suggesting that this protein modification is a new regulatory component in these pathways.
引用
收藏
页码:4915 / 4927
页数:13
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