CHARACTERIZATION AND CROSS APPLICATION OF NOVEL MICROSATELLITE MARKERS FOR A RARE SEDGE, LEPIDOSPERMA GIBSONII (CYPERACEAE)

被引：6
作者：
Barrett, Matthew D. [1 ,2 ,3 ]
Wallace, Mark J. [1 ,2 ,3 ]
Anthony, Janet M. [1 ,2 ,3 ]
机构：
[1] Bot Gardens, Perth, WA 6005, Australia
[2] Pk Author, Perth, WA 6005, Australia
[3] Univ Western Australia, Fac Nat & Agr Sci, Sch Plant Biol, Crawley, WA 6009, Australia
来源：
AMERICAN JOURNAL OF BOTANY | 2012年 / 99卷 / 01期
关键词：
clonality; Cyperaceae; genetic variation; hybridization; Lepidosperma gibsonii; microsatellite; simple sequence repeat markers;
D O I：
10.3732/ajb.1100357
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Premise of the study: Ten polymorphic microsatellite loci for the rare sword sedge Lepidosperma gibsonii (Cyperaceae) were characterized for the future study of population structure, hybridization, and clonality. Methods and Results: Twenty samples from each of three populations were screened with the markers to assess genetic variation. Observed population heterozygosities ranged from 0.35 to 1.00, and number of alleles observed per locus ranged from eight to 23. No departures from Hardy-Weinberg equilibrium were detected for any locus in any population. Single samples from 14 species were screened to examine the transferability of the microsatellites to other species of Lepidosperma. At least eight out of 10 loci amplified in all species tested. Conclusions: These loci will be useful for studying genetic variation, hybridization, dispersal, and breeding systems in Lepidosperma, a ubiquitous element of the flora of southern Australia.
引用
收藏
页码：E14 / E16
页数：3
相关论文
共 7 条
[1]  
Barrett Russell L., 2007, Nuytsia, V17, P37
[2]   SEGREGATION OF RANDOM AMPLIFIED DNA MARKERS IN F1 PROGENY OF CONIFERS [J].
CARLSON, JE ;
TULSIERAM, LK ;
GLAUBITZ, JC ;
LUK, VWK ;
KAUFFELDT, C ;
RUTLEDGE, R .
THEORETICAL AND APPLIED GENETICS, 1991, 83 (02) :194-200
[3]   Systematic studies in Lepidosperma (Cyperaceae: Schoeneae) with particular reference to L-laterale [J].
Hodgon, John ;
Bruhl, Jeremy J. ;
Wilson, Karen L. .
AUSTRALIAN SYSTEMATIC BOTANY, 2006, 19 (03) :273-288
[4]   Characterization of 11 polymorphic tetranucleotide microsatellites for forensic applications in California elk (Cervus elaphus canadensis) [J].
Jones, KC ;
Levine, KF ;
Banks, JD .
MOLECULAR ECOLOGY NOTES, 2002, 2 (04) :425-427
[5]   GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research [J].
Peakall, R ;
Smouse, PE .
MOLECULAR ECOLOGY NOTES, 2006, 6 (01) :288-295
[6]  
Rozen S, 2000, Methods Mol Biol, V132, P365
[7]   Novel chloroplast markers for the study of intraspecific variation and hybridisation in the Lepidosperma costale species complex (Cyperaceae) [J].
Wallace, Mark J. ;
Barrett, Matthew D. ;
Barrett, Russell L. .
CONSERVATION GENETICS RESOURCES, 2011, 3 (02) :355-360
1