Loop-mediated isothermal amplification (LAMP) for the rapid detection of Mycoplasma genitalium

被引：17

作者：

Edwards, Thomas ^{[1
]}

Burke, Patricia ^{[1
]}

Smalley, Helen B. ^{[1
]}

Gillies, Liz ^{[2
]}

Longhurst, Denise ^{[3
]}

Vipond, Barry ^{[3
]}

Hobbs, Glyn ^{[1
]}

机构：

[1] Liverpool John Moores Univ, Sch Pharm & Biomol Sci, Liverpool L3 5UX, Merseyside, England

[2] Mast Grp Ltd, Liverpool, Merseyside, England

[3] Publ Hlth England Bristol Lab, Specialist Virol Ctr, Bristol, Avon, England

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2015年 / 83卷 / 01期

关键词：

Mycoplasma genitalium; Loop mediated isothermal amplification; Diagnostic; PCR; Urethritis; Rapid testing; REAL-TIME PCR; QUANTITATIVE DETECTION; ASSAY; URETHRITIS; QUANTIFICATION; URINE; MEN; DNA;

D O I：

10.1016/j.diagmicrobio.2015.05.010

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Mycoplasma genitalium is a sexually transmissible, pathogenic bacterium and a significant cause of nongonococcal urethritis in both men and women. Due to the difficulty of the culture of M. genitalium from clinical samples, the laboratory diagnosis of M. genitalium infection is almost exclusively carried out using nucleic acid amplification tests. Loop-mediated isothermal amplification (LAMP) is a novel nucleic acid amplification technology, utilising a set of 4 primers specific to 6 distinct regions of the target DNA sequence, in order to amplify target DNA in a highly specific and rapid manner. A LAMP assay was designed to the pdhD gene of M. genitalium, and the limit of detection of the assay was determined as 10 fg of M. genitalium genomic DNA. equating to similar to 16 copies of the M. genitalium genome, which was equally sensitive as a gold standard 16S rRNA polymerase chain reaction assay. (C) 2015 Elsevier Inc. All rights reserved.

引用

页码：13 / 17

页数：5

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