Synergistic stimulating effect of 2-hydroxymelatonin and BMP-4 on osteogenic differentiation in vitro

被引：4
作者：
Lee, Sung Ho [1 ]
Hwang, Jin Wook [2 ,3 ]
Han, Younho [4 ]
Lee, Kwang Youl [5 ,6 ]
机构：
[1] Baylor Coll Med, Dept Mol & Cellular Biol, Houston, TX 77030 USA
[2] Univ Paris Sud, UMR S 935, INSERM, F-94800 Villejuif, France
[3] Univ Paris Sud, ESTeam Paris Sud, F-94800 Villejuif, France
[4] Wonkwang Univ, Coll Dent, Dept Oral Pharmacol, Iksan, South Korea
[5] Chonnam Natl Univ, Coll Pharm, Gwangju 500757, South Korea
[6] Chonnam Natl Univ, Res Inst Drug Dev, Gwangju 500757, South Korea
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2020年 / 527卷 / 04期
关键词：
2-Hydroxymelatonin; BMP-4; Osteoblast differentiation; OSTEOBLAST DIFFERENTIATION; MELATONIN; PHOSPHORYLATION; BONE; EXPRESSION; RUNX2; MINERALIZATION; PATHWAYS; CELLS; CREB;
D O I：
10.1016/j.bbrc.2020.04.126
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
2-Hydroxymelatonin is a metabolite produced when melatonin 2-hydroxylase catalyzes melatonin. Recent studies have reported the important roles of melatonin in bone metabolism. However, the roles of 2-hydroxymelatonin in bone metabolism remains poorly understood. The purpose of this study is to present evidence of the effect of 2-hydroxymelatonin on osteogenic differentiation in C2C12 cells. In this study, we demonstrated the synergistic stimulating effect of 2-hydroxymelatonin and bone morphogenetic protein (BMP)-4 on osteogenic differentiation in vitro, using alkaline phosphatase (ALP) staining, Alizarin red S (ARS) staining, qPCR, and luciferase reporter assay. The combination of 2hydroxymelatonin and BMP-4 revealed a synergistic effect on osteogenic differentiation in vitro. This finding provides evidence that optimal concentrations of both 2-hydroxymelatonin and BMP-4 are beneficial for anabolic effects on bone in vitro. (C) 2020 Elsevier Inc. All rights reserved.
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收藏
页码：941 / 946
页数：6
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