Ultrasensitive staining-free protein detection after PAA gel electrophoresis using deep UV fluorescence

被引：0

作者：

Riaplov, Eugene ^{[1
]}

Li, Qiang ^{[1
]}

Seeger, Stefan ^{[1
]}

机构：

[1] Univ Zurich, Inst Phys Chem, CH-8057 Zurich, Switzerland

来源：

PROTEIN AND PEPTIDE LETTERS | 2007年 / 14卷 / 07期

关键词：

native fluorescence; deep-UV; polyacrylamide; gel electrophoresis; staining free;

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We present the observation of separated protein bands after polyacrylamide (PAA) gel electrophoresis based on the staining-free detection of their ultra violet (UV)-induced fluorescence employing deep UV confocal fluorescence microscopy. Mixtures of the three biological compounds Galactosidase (from Escherichia coli), apo-Transferrin (bovine) and bovine serum albumin (BSA) have been separated and a staining free detection limit below 80 pg (7.0 x 10(8) molecules) per band has been achieved. This corresponds to similar to 270 molecules in the detection volume for confocal microscopy.

引用

页码：712 / 715

页数：4

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