Enhancement of DNA double-strand break induction and cell killing by K-shell absorption of phosphorus in human cell lines

被引:4
作者
Tomita, Masanori [1 ]
Maeda, Munetoshi [1 ,2 ]
Usami, Noriko [3 ]
Yokoya, Akinari [4 ]
Watanabe, Ritsuko [4 ]
Kobayashi, Katsumi [3 ]
机构
[1] Cent Res Inst Elect Power Ind, Radiat Safety Res Ctr, 2-11-1 Iwado Kita, Komae, Tokyo 2018511, Japan
[2] Wakasa Wan Energy Res Ctr, Dept Res & Dev, Proton Med Res Grp, Tsuruga, Fukui, Japan
[3] High Energy Accelerator Res Org KEK, Photon Factory, Inst Mat Struct Sci, Tsukuba, Ibaraki, Japan
[4] Japan Atom Energy Agcy, Quantum Beam Sci Ctr, Res Grp Radiat & Biomol Sci, Tokai, Ibaraki, Japan
关键词
Soft X-rays; Auger effect; double-strand break; DNA repair; cell death; ATAXIA-TELANGIECTASIA GENE; MONOCHROMATIC X-RAYS; INTRACELLULAR PHOSPHORUS; SYNCHROTRON-RADIATION; RESONANCE ENERGY; MAMMALIAN-CELLS; PROTEIN-KINASE; DOSE-RESPONSE; PLASMID DNA; YEAST-CELLS;
D O I
10.1080/09553002.2016.1179811
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Purpose: To investigate an enhancement of DNA double-strand break (DSB) induction and cell killing effect by K-shell ionization of phosphorus atoms and Auger electrons on human cell lines.Materials and methods: Induction of DSB, DNA damage responses, cell cycle distributions, and cell killing effects were investigated after exposures of the cells with monochromatic synchrotron radiation soft X-rays of 2153 and 2147eV, which were the resonance peak and off peak, respectively, of the K-shell photoabsorption of phosphorus.Results: Higher biological effects in the cells irradiated with soft X-rays at 2153eV than at 2147eV were observed in (i) the efficiency of 53BP1/-H2AX co-localized foci formation per dose and residual number of foci, (ii) prolonged phosphorylation levels of DSB repair and/or cell cycle checkpoint related proteins and G2 arrest, (iii) the cell killing effects at the 10% survival level of normal human fibroblasts, HeLa cells, and human glioblastoma M059K cells (1.2-1.5 times higher) and that of human ataxia telangiectasia mutated (ATM)-defective cells and glioblastoma DNA-dependent protein kinase catalytic subunit (DNA-PKcs)-defective cells (1.2 times).Conclusion: The yield of DSB and partly less-reparable complex DNA damage induction in human cells was enhanced by K-shell photoabsorption of phosphorus and low-energy Auger electrons.
引用
收藏
页码:724 / 732
页数:9
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