Development and evaluation of a selective medium for Brucella suis

被引：11

作者：

Ferreira, Ana Cristina ^{[1
,6
]}

Almendra, Claudia ^{[2
,3
]}

Cardoso, Regina ^{[1
]}

Pereira, Marta Silva ^{[4
]}

Beja-Pereira, Albano ^{[2
]}

Luikart, Gordon ^{[2
,5
]}

Correa de Sa, Maria Inacia ^{[1
]}

机构：

[1] INRB, IP LNIV, Lab Nacl Invest Vet, P-1549011 Lisbon, Portugal

[2] Ctr Invest Biodiversidade & Recursos Genet, CIBIO, P-4485661 Vairao, Portugal

[3] Univ Porto, Inst Ciencias Biomed, ICBAS, P-4099003 Oporto, Portugal

[4] Fullsense, P-3460070 Tondela, Portugal

[5] Univ Montana, Flathead Lake Biol Stn, Polson, MT 59860 USA

[6] Univ Lisbon, Fac Ciencias, Ctr Biodiversidade Genomica Integrat & Func BioFI, P-1749016 Lisbon, Portugal

来源：

RESEARCH IN VETERINARY SCIENCE | 2012年 / 93卷 / 02期

基金：

美国国家科学基金会;

关键词：

Brucella suis; Wild boar; Selective medium; Bacteriological diagnosis; BOARS SUS-SCROFA; MELITENSIS; ABORTUS; GROWTH;

D O I：

10.1016/j.rvsc.2011.09.004

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

A new selective medium, named LNIV-M, has been developed for isolation of Brucella suis. In this work, we evaluated the growth of B. suis reference and field strains from domestic pigs in different basal media and the susceptibility to different antibiotics contained in the currently used Farrell's and modified Thayer-Martin media. We also determined the efficacy of LNIV-M and its diagnostic performance for isolating B. suis from wild boar tissue samples. A total of 1649 samples from 918 hunter-harvested wild boars were cultured in LNIV-M, Farrell's and modified Thayer-Martin media. One hundred and thirty-nine (8.4%) samples from 63 (6.9%) animals resulted in a positive culture. LNIV-M detected 93.6% and 62.6% of positive animals and samples, respectively, while Farrell's and modified Thayer-Martin media detected, respectively, 92.1% and 79.4% of positive animals and 58.3% and 59.7% of samples. These results confirm the adequate diagnostic performance of LNIV-M in the isolation of B. suis. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：565 / 567

页数：3

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