Contrasting apoptotic responses of conjugated linoleic acid in the liver of obese Zucker rats fed palm oil or ovine fat

被引：3

作者：

Lopes, Paula A. ^{[1
]}

Martins, Susana V. ^{[1
]}

Viana, Ricardo S. J. ^{[2
]}

Ramalho, Rita M. ^{[2
]}

Alfaia, Cristina M. ^{[1
]}

Pinho, Mario S. ^{[1
]}

Jeronimo, Eliana ^{[1
]}

Bessa, Rui J. B. ^{[1
]}

Castro, Matilde F. ^{[2
]}

Rodrigues, Cecilia M. P. ^{[2
]}

Prates, Jose A. M. ^{[1
]}

机构：

[1] Univ Tecn Lisboa, CIISA, Fac Med Vet, P-1300477 Lisbon, Portugal

[2] Univ Lisbon, Res Inst Med & Pharmaceut Sci iMed UL, Fac Farm, P-1649003 Lisbon, Portugal

来源：

PROSTAGLANDINS LEUKOTRIENES AND ESSENTIAL FATTY ACIDS | 2011年 / 85卷 / 02期

关键词：

Conjugated linoleic acid; Palm oil; Ovine fat; Apoptosis; Obese Zucker rat; ENDOPLASMIC-RETICULUM STRESS; NF-KAPPA-B; HEPATIC STEATOSIS; ADIPOSE-TISSUE; CELL-DEATH; BODY-FAT; HEPATOCYTE APOPTOSIS; LIPID-METABOLISM; INDUCE; EXPRESSION;

D O I：

10.1016/j.plefa.2011.04.026

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We hypothesized that reducing weight properties of conjugated linoleic acid (CLA) are due to adipocyte apoptosis and that CLA differentially modulates the apoptotic responses in hepatic lipotoxicity from rats fed saturated fat diets. Obese Zucker rats were fed atherogenic diets (2% w/w of cholesterol) formulated with high (15% w/w) saturated fat, from vegetable or animal origin, supplemented or not with 1% of a mixture (1:1) of cis-9, trans-11 and trans-10, cis-12 CLA isomers for 14 weeks. CLA induced no changes on retroperitoneal fat depot weight, which was in line with similar levels of apoptosis. Interestingly, CLA had a contrasting effect on cell death in the liver according to the dietary fat. CLA increased hepatocyte apoptosis, associated with upregulation of Fas protein in rats fed palm oil, compared to rats receiving palm oil alone. However, rats fed ovine fat alone displayed the highest levels of hepatic cell death, which were decreased in rats fed ovine fat plus CLA. This reducing effect of CLA was related to positively restoring endoplasmic reticulum (ER) ATF-6 alpha, BiP and CHOP protein levels and increasing phosphorylated c-Jun NH2-terminal kinase (JNK) and c-Jun, thus suggesting an adaptive response of cell survival. These findings reinforce the role of CLA as regulator of apoptosis in the liver. Moreover, the dietary fat composition is a key factor in activation of apoptosis. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：89 / 96

页数：8

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