Quercetin down-regulated bcl-2 gene expression in human leukemia HL-60 cells

被引：0
|
作者：
Xiao, D
Gu, ZL [1 ]
Zhu, SP
机构：
[1] Suzhou Med Coll, Dept Pharmacol, Suzhou 215007, Peoples R China
[2] Suzhou Med Coll, Dept Radiotoxicol, Suzhou 215007, Peoples R China
来源：
ACTA PHARMACOLOGICA SINICA | 1998年 / 19卷 / 06期
关键词：
HL-60; cells; quercetin; gene expression; bcl-2; genes; DNA damage; immunohistochemistry; immunoblotting;
D O I：
暂无
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
AIM: To study the effect of quercetin (Que) on bcl-2 gene expression in human leukemia HL-60 cells. METHODS: Immunohistochemical analysis and RNA Dot blot hybridization were used to identify the expression of bcl-2 genes. RESULTS: The expression of bcl-2 protein was 94% in control HL-60 cells, which became 45% - 84% when they were cultured with Que 15 - 60 mu mol . L-1 for 48 h. The expression of bcl-2 mRNA in HL-60 cells was obviously decreased in treatment with Que 15 - 60 mu mol . L-1. CONCLUSION: The apoptotic action of Que in HL-60 cells was associated with the downregulation of the apoptosis-suppressing gene bcl-2.
引用
收藏
页码：551 / 553
页数：3
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