Genetic Typing, Based on the 56-Kilodalton Type-Specific Antigen Gene, of Orientia tsutsugamushi Strains Isolated from Chiggers Collected from Wild-Caught Rodents in Taiwan

被引：24

作者：

Lin, Pey-Ru ^{[1
]}

Tsai, Hui-Ping ^{[1
]}

Tsui, Pei-Yi ^{[1
]}

Weng, Ming-Hui ^{[1
]}

Kuo, Ming-Der ^{[1
]}

Lin, Hung-Chi ^{[1
]}

Chen, Kuo-Ching ^{[1
]}

Ji, Dar-Der ^{[1
]}

Chu, Der-Ming ^{[1
]}

Liu, Wen-Tssann ^{[1
]}

机构：

[1] Natl Def Med Ctr, Inst Prevent Med, Taipei, Taiwan

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2011年 / 77卷 / 10期

关键词：

SCRUB TYPHUS; RICKETTSIA-TSUTSUGAMUSHI; RECOMBINANT ANTIGEN; SEQUENCE; ASSAY; EASTERN; CLONING; DNA;

D O I：

10.1128/AEM.02796-10

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Orientia tsutsugamushi is the etiological agent of scrub typhus, a mite-borne, febrile illness that occurs in the Asia-Pacific region. We conducted strain characterization of O. tsutsugamushi isolates from chiggers obtained from rodents based the nucleotide sequence of the 56-kDa outer membrane protein gene. With the use of PCR, a total of 68 DNA sequences of 56-kDa antigen genes were amplified. Phylogenetic analysis revealed that there were at least six definable clusters among the 68 isolates: 37% Karp-related strains (25/68), 27% TA763 strains (18/68), 12% JG-related strains (8/68), 19% Kato-related strains (13/68), 4% divergent strains (3/68), and 1% representing a Gilliam prototype strain (1/68). Overall, the O. tsutsugamushi genotypes exhibited a high degree of diversity, similar to that seen in strains from the rest of the areas where scrub typhus is endemic. Moreover, the 56-kDa protein sequence similarity between O. tsutsugamushi isolates from mites and those from human patients (H. Y. Lu et al., Am. J. Trop. Med. Hyg. 83:658-663, 2010) were striking, thus highlighting potential risk factors for this emerging zoonotic disease.

引用

页码：3398 / 3405

页数：8

共 33 条

[1] Genetic typing of the 56-kDa type-specific antigen gene of contemporary Orientia tsutsugamushi isolates causing human scrub typhus at two sites in north-eastern and western Thailand [J].