A rapid and inexpensive method to screen for common foods that reduce the action of acrylamide, a harmful substance in food

被引：21

作者：

Hasegawa, Koichi ^{[1
,2
]}

Miwa, Satsuki ^{[1
]}

Tajima, Tomoko ^{[3
]}

Tsutsumiuchi, Kaname ^{[1
,3
]}

Taniguchi, Hajime ^{[4
]}

Miwa, Johji ^{[1
,3
]}

机构：

[1] Chubu Univ, Inst Biol Funct, Kasugai, Aichi 4878501, Japan

[2] Kyoto Univ, Grad Sch Agr, Sakyo Ku, Kyoto 6068502, Japan

[3] Chubu Univ, Grad Sch Biosci & Biotechnol, Kasugai, Aichi 4878501, Japan

[4] Ishikawa Prefectural Univ, Dept Environm Bioresource, Nonoichi, Ishikawa 9218836, Japan

来源：

TOXICOLOGY LETTERS | 2007年 / 175卷 / 1-3期

关键词：

biosensor; acrylamide; GST; food; Caenorhabditis elegans;

D O I：

10.1016/j.toxlet.2007.09.013

中图分类号：

R99 [毒物学（毒理学）];

学科分类号：

100405 ;

摘要：

By DNA microarray and protein 2-DE screens for Caenorhabditis elegans genes up-regulated by acrylamide, we selected the gst-4 gene and constructed a gst::gfp fusion gene, which was used to transform C. elegans into a biosensor for acrylamide. This biosensor detects acrylamide as a GFP-expression signal in a dose- and time-dependent manner. When the biosensor was exposed to acrylamide together with commercially available powdered green tea, GFP levels decreased to the control level, suggestive of acrylamide detoxification or prevention of GST induction. The present methodology should be applicable for screening of not only harmful substances but also substances that reduce or counteract their harmfulness or action, with appropriately constructed visible biosensors. (C) 2007 Elsevier Ireland Ltd. All rights reserved.

引用

页码：82 / 88

页数：7

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