Valorization of agroindustrial wastes: Identification by LC-MS and NMR of benzylglucosinolate from papaya (Carica papaya L.) seeds, a protective agent against lipid oxidation in edible oils

被引：15

作者：

Castro-Vargas, Henry I. ^{[1
]}

Baumann, Wolfram ^{[2
]}

Parada-Alfonso, Fabian ^{[1
]}

机构：

[1] Univ Nacl Colombia, Dept Chem, Carrera 30 45-03, Bogota, DC, Colombia

[2] Univ Los Andes, Dept Chem, Bogota, Colombia

来源：

ELECTROPHORESIS | 2016年 / 37卷 / 13期

关键词：

Antioxidant activity; Benzylglucosinolate; Carica papaya L; Edible oil; Papaya seeds; TANDEM MASS-SPECTROMETRY; ANTIOXIDANT ACTIVITY; BENZYL GLUCOSINOLATE; ION-TRAP; SYNTHETIC ANTIOXIDANTS; CHLOROFORM EXTRACT; HYDROLYSIS PRODUCT; MEDICINAL-PLANTS; ISOTHIOCYANATE; BRASSICACEAE;

D O I：

10.1002/elps.201500499

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In the present study we report the characterization of benzylglucosinolate (BG) isolated from papaya (Carica papaya L.) seeds. A methanolic extract was fractionated and further purified by solid phase extraction (SPE). It was analyzed by liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS), and nuclear magnetic resonance spectroscopy (H-1 and C-13-NMR) as well, and the target compound BG was identified by these two techniques. The effect of BG on lipid oxidation in edible vegetable oil (EO) was shown by observing some lipid oxidation products (linoleic acid hydroperoxides, LHP; hexanal, HEX; nonanal, NON; thiobarbituric acid reactives species, TBARS). BG reduced lipid oxidation production in EO by over 80%, as compared to a control sample and in this way has proved to be a useful antioxidant, even more effective than some antioxidants used by food industry.

引用

页码：1930 / 1937

页数：8