Enhanced resolution through thick tissue with structured illumination and adaptive optics

被引：52

作者：

Thomas, Benjamin ^{[1
]}

Wolstenholme, Adrian ^{[2
]}

Chaudhari, Snehal N. ^{[3
]}

Kipreos, Edward T. ^{[3
]}

Kner, Peter ^{[1
]}

机构：

[1] Univ Georgia, Coll Engn, Athens, GA 30602 USA

[2] Univ Georgia, Coll Vet Med, Dept Infect Dis, Athens, GA 30602 USA

[3] Univ Georgia, Dept Cellular Biol, Athens, GA 30602 USA

来源：

JOURNAL OF BIOMEDICAL OPTICS | 2015年 / 20卷 / 02期

基金：

美国国家科学基金会;

关键词：

fluorescence microscopy; adaptive optics; structured illumination microscopy; superresolution microscopy; biological imaging; SPECIMEN-INDUCED ABERRATIONS; WIDE-FIELD MICROSCOPY; FLUORESCENCE MICROSCOPY; DIFFRACTION-LIMIT; C-ELEGANS; LIVE; DYNAMICS; CELLS;

D O I：

10.1117/1.JBO.20.2.026006

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Structured illumination microscopy provides twice the linear resolution of conventional fluorescence microscopy, but in thick samples, aberrations degrade the performance and limit the resolution. Here, we demonstrate structured illumination microscopy through 35 mu m of tissue using adaptive optics (AO) to correct aberrations resulting in images with a resolution of 140 nm. We report a 60% minimum improvement in the signal-to-noise ratio of the structured illumination reconstruction through thick tissue by correction with AO. (C) 2015 Society of Photo-Optical Instrumentation Engineers (SPIE)

引用

页数：8

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