Comparative Analysis of Genome Editors Efficiency on a Model of Mice Zygotes Microinjection

被引：3

作者：

Averina, Olga A. ^{[1
,2
]}

Permyakov, Oleg A. ^{[1
]}

Grigorieva, Olga O. ^{[1
]}

Starshin, Alexey S. ^{[3
]}

Mazur, Alexander M. ^{[3
]}

Prokhortchouk, Egor B. ^{[3
]}

Dontsova, Olga A. ^{[4
,5
,6
]}

Sergiev, Petr, V ^{[1
,4
,5
]}

机构：

[1] Lomonosov Moscow State Univ, Inst Funct Genom, Moscow 119991, Russia

[2] Lomonosov Moscow State Univ, Belozersky Inst Physicochem Biol, Moscow 119899, Russia

[3] Russian Acad Sci, Inst Bioengn, Res Ctr Biotechnol, Moscow 119071, Russia

[4] Lomonosov Moscow State Univ, Dept Chem, Moscow 119991, Russia

[5] Skolkovo Inst Sci & Technol, Ctr Life Sci, Moscow 121205, Russia

[6] Shemyakin Ovchinnikov Inst Bioorgan Chem, Moscow 117997, Russia

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2021年 / 22卷 / 19期

基金：

俄罗斯科学基金会;

关键词：

genome editing; CRISPR Cas9; knockout mice; microinjection; homologous recombination; prime editor; MUTATIONS;

D O I：

10.3390/ijms221910221

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Genome editing is an indispensable tool for functional genomics. The caveat of the genome-editing pipeline is a prevalence of error-prone non-homologous end joining over homologous recombination, while only the latter is suitable to introduce particularly desired genetic variants. To overcome this problem, a toolbox of genome engineering was appended by a variety of improved instruments. In this work, we compared the efficiency of a number of recently suggested improved systems for genome editing applied to the same genome regions on a murine zygote model via microinjection. As a result, we observed that homologous recombination utilizing an ssDNA template following sgRNA directed Cas9 cleavage is still the method of choice for the creation of animals with precise genome alterations.

引用

页数：10

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