Structural investigation of the capsular polysaccharide from a clinical isolate of Fusobacterium necrophorum subspecies necrophorum biotype a strain LA 81-617

被引：0

作者：

Vinogradov, Evgeny ^{[1
]}

Altman, Eleonora ^{[1
]}

机构：

[1] Natl Res Council Canada, Human Hlth Therapeut Ctr, Ottawa, ON K1A 0R6, Canada

来源：

CARBOHYDRATE RESEARCH | 2020年 / 487卷

关键词：

Fusobacterium necrophorum; Capsular polysaccharide structure; Peptidoglycan; N-acetylmuramic acid;

D O I：

10.1016/j.carres.2019.107876

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A capsular polysaccharide (CPS) from Fusobacterium necrophorum subspecies necrophorum biotype A strain LA 81-617 was isolated from a saline cell wash and purified by gel and anion-exchange chromatography. The structure of the CPS was studied by one- and two-dimensional H-1 and C-13 NMR spectroscopy techniques in combination with electrospray ionization mass spectrometry (ESI-MS). The CPS was found to resemble the bacterial cell-wall murein polysaccharide backbone, consisting of N-acetylglucosamine and N-acetylmuramic acid (MurNAc) components, with the following structure: [-4-beta-MurNAc-4-beta-GlcNAc-](n)-with N-acetyl-1,6-anhydro-beta-muramic acid at the reducing end. This is the first report on the structure of F. necrophorum capsular polysaccharide.

引用

页数：4

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