Development of an allele-specific marker for Glu-B3 alleles in common wheat and establishment of a multiplex PCR assay

被引：3

作者：

Sui, Xinxia ^{[1
,2
]}

Wang, Linhai ^{[1
]}

Xia, Xianchun ^{[1
]}

Wang, Zhenlin ^{[3
]}

He, Zhonghu ^{[1
,4
]}

机构：

[1] Chinese Acad Agr Sci, Inst Crop Sci, Natl Wheat Improvement Ctr, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China

[2] Shandong Acad Agr Sci, Crop Res Inst, Jinan 250100, Peoples R China

[3] Shandong Agr Univ, Coll Life Sci, Tai An 271018, Shandong, Peoples R China

[4] Chinese Acad Agr Sci, China Off, Int Maize & Wheat Improvement Ctr CIMMYT, Beijing 100081, Peoples R China

来源：

CROP & PASTURE SCIENCE | 2010年 / 61卷 / 12期

基金：

中国国家自然科学基金;

关键词：

Functional marker; LMW-GS; multiplex PCR; Triticum aestivum L; LOW-MOLECULAR-WEIGHT; TRITICUM-AESTIVUM L; GLUTENIN SUBUNIT GENES; CHINESE BREAD WHEATS; DOUGH PROPERTIES; STS MARKERS; QUALITY; LMW; PROTEINS; LOCI;

D O I：

10.1071/CP10241

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

Low-molecular-weight glutenin subunits (LMW-GS) have significant effects on the processing quality of enduse products of common wheat. It is more efficient to discriminate LMW-GSalleles with PCR-based molecular markers than with SDS-PAGE. In the present study, we developed an allele-specific PCR marker, designated Glu-B3abefg, which can be used to discriminate protein alleles Glu-B3a, b,e,f, and g simultaneously. Based on ten previously developed allele-specific STS markers, three multiplex PCRs, viz. Glu-B3c + Glu-B3d, Glu-B3b + Glu-B3g, and Glu-B3h + Glu-B3i, were established. Six Glu-B3 alleles (b, c, d, g, h, and i) could be discriminated using the three multiplex PCRs. Results of tests on 158 wheat varieties and lines using Glu-B3abefg and the three multiplex PCRs were consistent with those using the ten STS markers separately. The new allele-specific marker and three multiplex PCRs represent an efficient way to undertake marker-assisted selection of Glu-B3 alleles.

引用

页码：978 / 987

页数：10