A plasmid-based multigene expression system for mammalian cells

被引：50

作者：

Kriz, Andrijana ^{[1
]}

Schmid, Katharina ^{[1
]}

Baumgartner, Nadia ^{[1
]}

Ziegler, Urs ^{[2
]}

Berger, Imre ^{[3
]}

Ballmer-Hofer, Kurt ^{[1
]}

Berger, Philipp ^{[1
]}

机构：

[1] Paul Scherrer Inst, CH-5232 Villigen, Switzerland

[2] Univ Zurich, Ctr Microscopy & Image Anal, CH-8057 Zurich, Switzerland

[3] EMBL, F-38042 Grenoble 9, France

来源：

NATURE COMMUNICATIONS | 2010年 / 1卷

基金：

瑞士国家科学基金会;

关键词：

RECOMBINANT-DNA; VECTORS;

D O I：

10.1038/ncomms1120

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The introduction of heterologous genetic information, particularly of multiple genes, into mammalian cells is a key technology in contemporary experimental biological research. The coexpression of fluorescently tagged sensors is required to simultaneously analyse multiple parameters in living cells and the coexpression of several proteins is necessary to manipulate cell fate in stem cell biology. Current technologies for multigene expression in mammalian cells are inefficient, inflexible and time-consuming. In this paper we describe MultiLabel, a novel and highly efficient modular plasmid-based eukaryotic expression system. Independent expression vectors are assembled by a Cre/LoxP reaction into a plasmid with multiple expression cassettes. MultiLabel enables rapid construction of multigene expression vectors for the single-step creation of transiently or stably transfected mammalian cells.

引用

页数：6

共 12 条

[1]

Bieniossek C, 2009, NAT METHODS, V6, P447, DOI [10.1038/NMETH.1326, 10.1038/nmeth.1326]

[2] The fluorescent protein palette: tools for cellular imaging [J].