Sputum Leucine-Rich Alpha-2 Glycoprotein as a Marker of Airway Inflammation in Asthma

被引：48

作者：

Honda, Hiromi ^{[1
]}

Fujimoto, Minoru ^{[1
]}

Miyamoto, Shintaro ^{[2
]}

Ishikawa, Nobuhisa ^{[3
]}

Serada, Satoshi ^{[1
]}

Hattori, Noboru ^{[4
]}

Nomura, Shintaro ^{[5
]}

Kohno, Nobuoki ^{[4
]}

Yokoyama, Akihito ^{[2
]}

Naka, Tetsuji ^{[1
,6
]}

机构：

[1] Natl Inst Biomed Innovat Hlth & Nutr, Lab Immune Signal, Ibaraki, Osaka, Japan

[2] Kochi Univ, Dept Haematol & Resp Med, Nankoku, Kochi, Japan

[3] Hiroshima Prefectural Hosp, Dept Resp Med, Minami Ku, Hiroshima, Japan

[4] Hiroshima Univ, Grad Sch Biomed Sci, Dept Mol & Internal Med, Minami Ku, Hiroshima, Japan

[5] Nagahama Inst Biosci & Technol, Dept Anim Biosci, Nagahama, Shiga, Japan

[6] Kochi Univ, Kochi Med Sch, Integrated Ctr Adv Med Technol ICAM Tech, Div Translat Res, Nankoku, Kochi, Japan

来源：

PLOS ONE | 2016年 / 11卷 / 09期

关键词：

EPITHELIAL-CELLS; HUMAN-SERUM; PERIOSTIN; ALPHA-2-GLYCOPROTEIN; DIFFERENTIATION; EXPRESSION; BIOMARKER; ALPHA(2)-GLYCOPROTEIN; ROLES; IL-13;

D O I：

10.1371/journal.pone.0162672

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Background Asthma is a chronic inflammatory disease of airways, but an ideal biomarker that accurately reflects ongoing airway inflammation has not yet been established. The aim of this study was to examine the potential of sputum leucine-rich alpha-2 glycoprotein (LRG) as a new biomarker for airway inflammation in asthma. Methods We obtained induced sputum samples from patients with asthma (N = 64) and healthy volunteers (N = 22) and measured LRG concentration by sandwich enzyme-linked immunosorbent assay (ELISA). Ovalbumin (OVA)-induced asthma model mice were used to investigate the mechanism of LRG production during airway inflammation. The LRG concentrations in the bronchoalveolar lavage fluid (BALF) obtained from mice were determined by ELISA and mouse lung sections were stained with anti-LRG antibody and periodic acid-Schiff (PAS) reagent. Results Sputum LRG concentrations were significantly higher in patients with asthma than in healthy volunteers (p = 0.00686). Consistent with patients' data, BALF LRG levels in asthma model mice were significantly higher than in control mice (p = 0.00013). Immunohistochemistry of lung sections from asthma model mice revealed that LRG was intensely expressed in a subpopulation of bronchial epithelial cells, which corresponded with PAS-positive mucus producing cells. Conclusion These findings suggest that sputum LRG is a promising biomarker of local inflammation in asthma.

引用

页数：12

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