Structure of the O-specific polysaccharide isolated from the lipopolysaccharide of Citrobacter gillenii serotype O12a,12b strain PCM 1544

被引:4
作者
Kübler-Kielb, J
Zatonsky, GV
Katzenellenbogen, E
Kocharova, NA
Szostko, B
Gamian, A
Shashkov, AS
Knirel, YA
机构
[1] Polish Acad Sci, L Hirszfeld Inst Immunol & Expt Therapy, PL-53114 Wroclaw, Poland
[2] Russian Acad Sci, ND Zelinsky Inst Organ Chem, Moscow 117913, Russia
基金
俄罗斯基础研究基金会;
关键词
Citrobacter; enterobacteria; O-antigen; lipopolysaccharide; structure;
D O I
10.1016/S0008-6215(01)00043-X
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A neutral O-specific polysaccharide was isolated from the lipopolysaccharide of Citrobacter gillenii strain PCM 1544, representing serotype O12a,12b. The polysaccharide was studied by sugar and methylation analyses and Smith degradation along with H-1 and C-13 NMR spectroscopy, including a ROESY experiment. The following structure of the tetrasaccharide repeating unit was established, in which substitution with terminal GlcNAc is similar to 60%. [GRAPHICS] (C) 2001 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:331 / 336
页数:6
相关论文
共 50 条
  • [31] NMR spectroscopic elucidation of the structure of a glycerol teichoic acid-like O-specific polysaccharide of the bacterium Hafnia alvei strain PCM 1199
    Zatonskii, GV
    Kocharova, NA
    Katzenellenbogen, E
    Shashkov, AS
    Knirel, YA
    BIOCHEMISTRY-MOSCOW, 1997, 62 (12) : 1448 - 1453
  • [32] O-specific polysaccharide structure of the aqueous lipopolysaccharide fraction from Xanthomonas campestris pv. vitians strain 1839
    Molinaro, A
    Evidente, A
    Lanzetta, R
    Parrilli, M
    Zoina, A
    CARBOHYDRATE RESEARCH, 2000, 328 (03) : 435 - 439
  • [33] Structure of a glycerol teichoic acid-like O-specific polysaccharide of Proteus vulgaris O12
    Perepelov, AV
    Torzewska, A
    Shashkov, AS
    Senchenkova, SN
    Rozalski, A
    Knirel, YA
    EUROPEAN JOURNAL OF BIOCHEMISTRY, 2000, 267 (03): : 788 - 793
  • [34] THE STRUCTURE OF THE O-SPECIFIC POLYSACCHARIDE OF THE LIPOPOLYSACCHARIDE FROM CHROMOBACTERIUM-VIOLACEUM NCTC-9694
    VINOGRADOV, EV
    BRADE, H
    HOLST, O
    CARBOHYDRATE RESEARCH, 1994, 264 (02) : 313 - 317
  • [35] The O-specific polysaccharide structure from the lipopolysaccharide of the Gram-negative bacterium Raoultella terrigena
    Leone, Serena
    Molinaro, Antonio
    Dubery, Ian
    Lanzetta, Rosa
    Parrilli, Michelangelo
    CARBOHYDRATE RESEARCH, 2007, 342 (11) : 1514 - 1518
  • [36] The identity of the O-specific polysaccharide structure of Citrobacter strains from serogroups O2, O20 and O25 and immunochemical characterisation of C-youngae PCM 1507 (O2a,1b) and related strains
    Mieszala, M
    Lipiniski, T
    Kocharova, NA
    Zatonsky, GV
    Katzenellenbogen, E
    Shashkov, AS
    Gamian, A
    Knirel, YA
    FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY, 2003, 36 (1-2): : 71 - 76
  • [37] THE STRUCTURE OF THE CITROBACTER-FREUNDII O8A,8B O-SPECIFIC POLYSACCHARIDE CONTAINING D-XYLOFURANOSE
    KOCHAROVA, NA
    KNIREL, YA
    SHASHKOV, AS
    KOCHETKOV, NK
    KHOLODKOVA, EV
    STANISLAVSKY, ES
    CARBOHYDRATE RESEARCH, 1994, 263 (02) : 327 - 331
  • [38] Structural and serological studies on a new 4-deoxy-D-arabino-hexose-containing O-specific polysaccharide from the lipopolysaccharide of Citrobacter braakii PCM 1531 (serogroup O6)
    Katzenellenbogen, E
    Kocharova, NA
    Zatonsky, GV
    Witkowska, D
    Bogulska, M
    Shashkov, AS
    Gamian, A
    Knirel, YA
    EUROPEAN JOURNAL OF BIOCHEMISTRY, 2003, 270 (13): : 2732 - 2738
  • [39] Structure of the sialic acid-containing O-specific polysaccharide from Salmonella enterica serovar Toucra O48 lipopolysaccharide
    Gamian, A
    Jones, C
    Lipinski, T
    Korzeniowska-Kowal, A
    Ravenscroft, N
    EUROPEAN JOURNAL OF BIOCHEMISTRY, 2000, 267 (11): : 3160 - 3166
  • [40] Revised structure of the repeating unit of the O-specific polysaccharide from Azospirillum lipoferum strain SpBr17
    Choma, Adam
    Komaniecka, Iwona
    Sowinski, Pawel
    CARBOHYDRATE RESEARCH, 2009, 344 (07) : 936 - 939