Structure of the O-specific polysaccharide isolated from the lipopolysaccharide of Citrobacter gillenii serotype O12a,12b strain PCM 1544

被引:4
|
作者
Kübler-Kielb, J
Zatonsky, GV
Katzenellenbogen, E
Kocharova, NA
Szostko, B
Gamian, A
Shashkov, AS
Knirel, YA
机构
[1] Polish Acad Sci, L Hirszfeld Inst Immunol & Expt Therapy, PL-53114 Wroclaw, Poland
[2] Russian Acad Sci, ND Zelinsky Inst Organ Chem, Moscow 117913, Russia
基金
俄罗斯基础研究基金会;
关键词
Citrobacter; enterobacteria; O-antigen; lipopolysaccharide; structure;
D O I
10.1016/S0008-6215(01)00043-X
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A neutral O-specific polysaccharide was isolated from the lipopolysaccharide of Citrobacter gillenii strain PCM 1544, representing serotype O12a,12b. The polysaccharide was studied by sugar and methylation analyses and Smith degradation along with H-1 and C-13 NMR spectroscopy, including a ROESY experiment. The following structure of the tetrasaccharide repeating unit was established, in which substitution with terminal GlcNAc is similar to 60%. [GRAPHICS] (C) 2001 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:331 / 336
页数:6
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