Macrophages control the retention and trafficking of B lymphocytes in the splenic marginal zone

被引:195
作者
Karlsson, MCI
Guinamard, R
Bolland, S
Sankala, M
Steinman, RM
Ravetch, JV
机构
[1] Rockefeller Univ, Lab Mol Genet & Immunol, New York, NY 10021 USA
[2] Rockefeller Univ, Cellular Physiol & Immunol Lab, New York, NY 10021 USA
[3] Univ Mediterranee, INSERM, CNRS, Ctr Immunol Marseille Luminy, F-13288 Marseille, France
[4] NIAID, Immunogenet Lab, NIH, Rockville, MD 20852 USA
[5] Karolinska Inst, Dept Med Biochem & Biophys, Div Matrix Biol, S-17177 Stockholm, Sweden
关键词
SHIP; Btk; MARCO; migration; Staphylococcus aureus;
D O I
10.1084/jem.20030684
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The marginal zone of the spleen is a precisely ordered region that contains specialized subsets of B lymphocytes and macrophages. Disruption of the negative signaling inositol phosphatase, SH2-containing mositol-5-phosphatase 1 (SHIP), results in the loss of marginal zone B cells (MZBs) with reorganization of marginal zone macrophages (MZMOs) to the red pulp of the spleen. This primary macrophage defect, as revealed by selectively depleting SHIP in myeloid cells shows that MZMOs are specifically required for the retention of MZBs. The MZMO phenotype was reverted in SHIP/Bruton's tyrosine kinase (Btk) double knockout mice, thus identifying the Btk activating pathway as an essential component being regulated by SHIP. Furthermore, we identified a direct interaction between the MARCO scavenger receptor on MZMOs and MZBs. Activation or disruption of this interaction results in MZB migration to the follicle. The migration of the MZMOs was further studied after the response to Staphylococcus aureus, which induced MZMOs to move into the red pulp while MZBs migrated into the follicular zone. The marginal zone is therefore a dynamic structure in which retention and trafficking of B cells requires specific macrophage-B cell interactions.
引用
收藏
页码:333 / 340
页数:8
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