Surface plasmon resonance Spectroscopy as a tool to study polyplex-glycoaminoglycan interactions

被引：5
作者：
Dubruel, P
Urtti, A
Schacht, E [1 ]
机构：
[1] State Univ Ghent, Dept Organ Chem, Polymer Mat Res Grp, B-9000 Ghent, Belgium
[2] Univ Kuopio, Dept Pharmaceut, FIN-70211 Kuopio, Finland
[3] State Univ Ghent, IBITECH, Inst Biomed Technol, B-9000 Ghent, Belgium
来源：
MACROMOLECULAR RAPID COMMUNICATIONS | 2005年 / 26卷 / 12期
关键词：
biological applications of polymers; gene therapy; polyelectrolytes; self-assembly; surface plasmon resonance;
D O I：
10.1002/marc.200500122
中图分类号：
O63 [高分子化学（高聚物）];
学科分类号：
070305 ; 080501 ; 081704 ;
摘要：
This article reports the application of surface plasmon resonance (SPR) to monitor the interaction between polymer-DNA complexes and glycoaminoglycans (GAG). The GAG selected was hyaluronic acid (HA). First a HA derivative containing a disulfide linkage was synthesized, enabling chemisorption onto a gold surface. Next, the interaction between different complexes (prepared using PEI or PDMAEMA) and HA was studied using SPR. This study clearly indicates that GAG-pollyplex interactions depend on the type of polymer selected and on the charge ratio of the polyplexes prepared.
引用
收藏
页码：992 / 997
页数：6
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