Investigation into potential mechanisms of metabolic syndrome by integrative analysis of metabolomics and proteomics

被引:12
作者
Chen, Meimei [1 ,2 ,3 ]
Yang, Zhaoyang [1 ,2 ,3 ]
Gan, Huijian [1 ,2 ,3 ]
Wang, Yang [1 ,2 ,3 ]
Li, Chandong [1 ,2 ,3 ]
Gao, Yuxing [4 ]
机构
[1] Fujian Univ Tradit Chinese Med, Coll Tradit Chinese Med, Fuzhou, Fujian, Peoples R China
[2] Fujian Univ Tradit Chinese Med, Fujian Key Lab TCM Hlth Status Identificat, Fuzhou, Fujian, Peoples R China
[3] Fujian Univ Tradit Chinese Med, Engn Res Ctr, Fuzhou, Fujian, Peoples R China
[4] Xiamen Univ, Coll Chem & Chem Engn, Xiamen, Fujian, Peoples R China
来源
PLOS ONE | 2022年 / 17卷 / 07期
关键词
D O I
10.1371/journal.pone.0270593
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Metabolic syndrome (MetS) is a complex syndrome cluster of metabolic disorders, which greatly increases the risks of diabetic and cardiovascular diseases. Although it has become a significantly worldwide public health burden, its pathogenesis largely remains unknown. In this study, we first performed an integrated analysis of proteomic and metabonomic data of liver tissues of rats between MetS and control groups to reveal possible mechanisms of MetS. A total of 16 significantly perturbed pathways were identified, of which three pathways were shared by patients with MetS and diabetes identified by analysis of serum samples, including alanine, aspartate and glutamate metabolism, valine, leucine and isoleucine biosynthesis, and glycine, serine and threonine metabolism. Additionally, it was found that 18 differential metabolites were closely related with 36 differential proteins, which were considered as significantly discriminant metabolites and proteins between two groups and were mainly involved in metabolic processes of gamma-aminobutyric acid and acetyl-CoA, biosynthetic processes of cholesterol and amino acids. The results of PPI network analysis and topological parameter calculation of four methods revealed that 16 proteins can serve as hub proteins of MetS. Followed by searching the PubMed database and molecular docking of Cyp7a1 and Got1, we concluded that atorvastatin and resveratrol may be potential drugs for MetS.
引用
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页数:17
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