Viroid-derived small RNA induces early flowering in tomato plants by RNA silencing

被引：33

作者：

Adkar-Purushothama, Charith Raj ^{[1
,2
]}

Sano, Teruo ^{[3
]}

Perreault, Jean-Pierre ^{[1
]}

机构：

[1] Univ Sherbrooke, RNA Grp, Grp ARN, Dept Biochim,Fac Med Sci Sante, Pavillon Rech Appl Canc,3201 Rue Jean Mignault, Sherbrooke, PQ J1E 4K8, Canada

[2] MYM Nutraceut Inc, 1500-409 Granville St, Vancouver, BC V6C 1T2, Canada

[3] Hirosaki Univ, Fac Agr & Life Sci, Hirosaki, Aomori 0368561, Japan

来源：

MOLECULAR PLANT PATHOLOGY | 2018年 / 19卷 / 11期

基金：

加拿大自然科学与工程研究理事会; 日本学术振兴会;

关键词：

early flowering; FRIGIDA-like protein 3; host-pathogen interaction; PSTVd; RNA silencing; vd-sRNA; viroid; TIME-VARIATION; ARABIDOPSIS; EXPRESSION; FRIGIDA; GENE; CONSTRUCTION; MUTATIONS; INFECTION; PATHWAYS; FLC;

D O I：

10.1111/mpp.12721

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Viroid infection often leads to early flowering in the host plant. This report describes the targeting of the FRIGIDA-like protein 3 (FRL3) mRNA in tomato plants by a small RNA derived from the conserved left terminal region of the potato spindle tuber viroid (PSTVd). This targeting leads to the silencing of the FRL3 mRNA. Viroid infection assays using a severe variant of PSTVd induced early flowering in tomato plants by the down-regulation of greater amounts of the target than did a mild PSTVd variant. The targeting of the FRL3 mRNA by RNA silencing was validated by both an artificial microRNA experiment transiently expressing viroid-derived small RNAs in tomato plants, and by 5 ' RNA ligase-mediated rapid amplification of cDNA ends (RACE). These data unambiguously demonstrated the role of small RNAs in the early flowering seen in viroid-infected plants.

引用

页码：2446 / 2458

页数：13

共 38 条

[1] Alterations of the viroid regions that interact with the host defense genes attenuate viroid infection in host plant [J].