A genetic system to assess in vivo the functions of histones and histone modifications in higher eukaryotes

被引：81

作者：

Guenesdogan, Ufuk ^{[1
]}

Jaeckle, Herbert ^{[1
]}

Herzig, Alf ^{[1
]}

机构：

[1] Max Planck Inst Biophys Chem, Abt Mol Entwicklungsbiol, D-37077 Gottingen, Germany

来源：

EMBO REPORTS | 2010年 / 11卷 / 10期

关键词：

histone deletion; histone transgenes; functional assay; Drosophila melanogaster; CYCLIN-B; CHROMATIN; EXPRESSION; PROTEIN;

D O I：

10.1038/embor.2010.124

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Despite the fundamental role of canonical histones in nucleosome structure, there is no experimental system for higher eukaryotes in which basic questions about histone function can be directly addressed. We developed a new genetic tool for Drosophila melanogaster in which the canonical histone complement can be replaced with multiple copies of experimentally modified histone transgenes. This new histone-replacement system provides a well-defined and direct cellular assay system for histone function with which to critically test models in chromatin biology dealing with chromatin assembly, variant histone functions and the biological significance of distinct histone modifications in a multicellular organism.

引用

页码：772 / 776

页数：5

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