A new water-soluble near-neutral ratiometric fluorescent pH indicator

被引：120
作者：
Yao, Sheng
Schafer-Hales, Katherine J.
Belfield, Kevin D.
机构：
[1] Univ Cent Florida, Coll Opt & Photon, CREOL, Dept Chem, Orlando, FL 32816 USA
[2] Emory Univ, Sch Med, Winship Canc Inst, Atlanta, GA 30322 USA
来源：
ORGANIC LETTERS | 2007年 / 9卷 / 26期
关键词：
D O I：
10.1021/ol7026366
中图分类号：
O62 [有机化学];
学科分类号：
070303 ; 081704 ;
摘要：
Donor-pi-acceptor fluorene derivative 1c is a near-neutral pH indicator whose pK(a) of similar to 7.0 was determined by both absorption and fluorescence methods. 1c satisfies important criteria for a sensitive ratiomeric fluorescent pH indicator with a distinctive isoemissive point, good dispersion in cell cytosol, and low cytotoxicity. Furthermore, its 2PA cross section of 100 GM in its neutral form suggests its. potential in two-photon fluorescence imaging applications.
引用
收藏
页码：5645 / 5648
页数：4
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