Differential regulation of exocytosis by calcium and CAPS in semi-intact synaptosomes

被引：108

作者：

Tandon, A

Bannykh, S

Kowalchyk, JA

Banerjee, A

Martin, TFJ

Balch, WE ^{[1
]}

机构：

[1] Scripps Res Inst, Dept Cell Biol, La Jolla, CA 92037 USA

[2] Scripps Res Inst, Dept Mol Biol, La Jolla, CA 92037 USA

[3] Univ Wisconsin, Dept Biochem, Madison, WI 53706 USA

[4] Univ Wisconsin, Dept Food Microbiol & Toxicol, Madison, WI 53706 USA

来源：

NEURON | 1998年 / 21卷 / 01期

关键词：

D O I：

10.1016/S0896-6273(00)80522-X

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Using a novel approach to measure exocytosis in vitro from semi-intact synaptosomes, we establish that the Ca2+-dependent release of glutamate requires cytosolic factors for mobilization from the reserve pool. The cytosolic activity for glutamate release was not satisfied by CAPS, a soluble component required for norepinephrine (NE) release. Moreover, the CAPS-independent glutamate release from synaptic vesicles (SVs) was 200-fold less sensitive to Ca2+ than that required for dense core vesicles (DCVs). The differential regulation of exocytosis by CAPS, Ca2+, and potential novel cytosolic factor(s) suggests that the docking and fusion machinery controlling DCVs has diverged from that regulating glutamate-containing SVs.

引用

页码：147 / 154

页数：8

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[1] INTRODUCTION OF MACROMOLECULES INTO BOVINE ADRENAL-MEDULLARY CHROMAFFIN CELLS AND RAT PHEOCHROMOCYTOMA CELLS (PC12) BY PERMEABILIZATION WITH STREPTOLYSIN-O - INHIBITORY EFFECT OF TETANUS TOXIN ON CATECHOLAMINE SECRETION [J].